About: Incorporation of beta-(1,6)-linked glucooligosaccharides (pustulooligosaccharides) into plant cell wall structures

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.2478/s11696-012-0167-x
Description	Protein extract of germinating nasturtium (Tropaeolum majus) seeds containing xyloglucan endotransglycosylase (xyloglucan xyloglucosyl transferase, EC 2.4.1.207, abbreviated XET) exhibited the heterotransglycosylating activity with donor/acceptor substrate pair xyloglucan/sulphorhodamine labelled pustulooligosaccharides (XG/PUOS-SR) in a dot blot assay. The heterotransglycosylating activity was confirmed by the substrate-product changes during transglycosylation by HPLC size-exclusion chromatography. Another donor substrate capable of being coupled with PUOS-SR was cellulose, probably owing to its structural similarity to xyloglucan. Surprisingly, microscopic comparison of the incorporation of the labelled xyloglucan nonasaccharide XGO9-SR (specific substrate for XET) and PUOS-SR into the cell wall structures clearly showed differences in their binding to specific cell structures: the primary cell wall and the plasma membrane. These findings indicate the existence in nasturtium of XETs with different localisation, substrate specificity and, probably, function. Protein extract of germinating nasturtium (Tropaeolum majus) seeds containing xyloglucan endotransglycosylase (xyloglucan xyloglucosyl transferase, EC 2.4.1.207, abbreviated XET) exhibited the heterotransglycosylating activity with donor/acceptor substrate pair xyloglucan/sulphorhodamine labelled pustulooligosaccharides (XG/PUOS-SR) in a dot blot assay. The heterotransglycosylating activity was confirmed by the substrate-product changes during transglycosylation by HPLC size-exclusion chromatography. Another donor substrate capable of being coupled with PUOS-SR was cellulose, probably owing to its structural similarity to xyloglucan. Surprisingly, microscopic comparison of the incorporation of the labelled xyloglucan nonasaccharide XGO9-SR (specific substrate for XET) and PUOS-SR into the cell wall structures clearly showed differences in their binding to specific cell structures: the primary cell wall and the plasma membrane. These findings indicate the existence in nasturtium of XETs with different localisation, substrate specificity and, probably, function. (en)
Title	Incorporation of beta-(1,6)-linked glucooligosaccharides (pustulooligosaccharides) into plant cell wall structures Incorporation of beta-(1,6)-linked glucooligosaccharides (pustulooligosaccharides) into plant cell wall structures (en)
skos:prefLabel	Incorporation of beta-(1,6)-linked glucooligosaccharides (pustulooligosaccharides) into plant cell wall structures Incorporation of beta-(1,6)-linked glucooligosaccharides (pustulooligosaccharides) into plant cell wall structures (en)
skos:notation	RIV/60162694:G44__/12:43874727!RIV13-MO0-G44_____
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z40310501), Z(MO0FVZ0000501)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Řehulka, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	141098
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/12:43874727
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	plasma membrane; cell wall; heterotransglycosylation; XET; xyloglucan endotransglycosylase (en)
http://linked.open.../riv/klicoveSlovo	plasma membrane cell wall XET heterotransglycosylation xyloglucan endotransglycosylase
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[9F8A36277851]
http://linked.open...i/riv/nazevZdroje	Chemical Papers-Chemicke Zvesti
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	66
http://linked.open...iv/tvurceVysledku	Flodrová, Dana Stratilová, Eva Garajová, Soňa Řehulka, Pavel Farkaš, Vladimír Vadkertiová, Renáta Zelko, Ivan Zemková, Zuzana
http://linked.open...ain/vavai/riv/wos	000305563300003
http://linked.open...n/vavai/riv/zamer	Moderní analytické techniky pro bioanalýzu, ekologii a nanotechnologie Zdravotnická problematika ZHN
issn	0366-6352
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.2478/s11696-012-0167-x
http://localhost/t...ganizacniJednotka	G44
is http://linked.open...avai/riv/vysledek of	Incorporation of beta-(1,6)-linked glucooligosaccharides (pustulooligosaccharides) into plant cell wall structures

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