About: LPS structure influences protein secretion in Salmonella enteritica

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.vetmic.2011.04.018
Description	In this study we have compared protein secretion in the wild type of S. Typhimurium and the rfaC mutant. We found out that the rfaC mutant was defective in protein secretion. In addition, the rfaC mutant was defective in its invasion into an IPEC-J2 porcine epithelial cell line and also in motility in semisolid agar. Consistent with this, reduced flagella numbers were observed in the rfaC mutant. In the rfaC mutant, there were no defects in flagellin expression as detected by western blot and immune electron microscopy which demonstrated equal amounts of flagellin in the cytoplasm of both the rfaC mutant and the wild-type S. Typhimurium. However, in the wild-type strain only, the flagellin was assembled to spatially restricted areas on the inner side of cytoplasmic membrane. The oligosaccharide core of LPS is therefore required for the assembly of flagella and T3SS secretion machinery followed by protein secretion. In this study we have compared protein secretion in the wild type of S. Typhimurium and the rfaC mutant. We found out that the rfaC mutant was defective in protein secretion. In addition, the rfaC mutant was defective in its invasion into an IPEC-J2 porcine epithelial cell line and also in motility in semisolid agar. Consistent with this, reduced flagella numbers were observed in the rfaC mutant. In the rfaC mutant, there were no defects in flagellin expression as detected by western blot and immune electron microscopy which demonstrated equal amounts of flagellin in the cytoplasm of both the rfaC mutant and the wild-type S. Typhimurium. However, in the wild-type strain only, the flagellin was assembled to spatially restricted areas on the inner side of cytoplasmic membrane. The oligosaccharide core of LPS is therefore required for the assembly of flagella and T3SS secretion machinery followed by protein secretion. (en)
Title	LPS structure influences protein secretion in Salmonella enteritica LPS structure influences protein secretion in Salmonella enteritica (en)
skos:prefLabel	LPS structure influences protein secretion in Salmonella enteritica LPS structure influences protein secretion in Salmonella enteritica (en)
skos:notation	RIV/60162694:G44__/11:00002556!RIV12-MO0-G44_____
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(ED0006/01/01), Z(MO0FVZ0000501), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Fučíková, Alena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	210052
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/11:00002556
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Salmonella; LPS; rfaC; protein secretion; motility; SPI-1 (en)
http://linked.open.../riv/klicoveSlovo	Salmonella LPS SPI-1 protein secretion rfaC motility
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C73D1CAC7D41]
http://linked.open...i/riv/nazevZdroje	Veterinary Microbiology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	152
http://linked.open...iv/tvurceVysledku	Karasová, Daniela Bortlicek, Zbyněk Crhanova, Magdaléna Děkanová, Michela Fučíková, Alena Kyrova, Kamila Malcova, Marcela Mazgajova, Monika Pilousová, Lenka Rychlik, Ivan Šebková, Alena
http://linked.open...ain/vavai/riv/wos	000294095000015
http://linked.open...n/vavai/riv/zamer	Zdravotnická problematika ZHN Animal disease research, prevention and food chain protection
issn	0378-1135
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	G44
is http://linked.open...avai/riv/vysledek of	LPS structure influences protein secretion in Salmonella enteritica

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