About: Francisella tularensis membrane complexome by blue native/SDS-PAGE

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Francisella tularensis membrane complexome by blue native/SDS-PAGE Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.jprot.2011.05.006
Description	The study of membrane proteins and membrane protein complexes (MPC) provides crucial information in the field of bacterial physiology and pathogenesis research. The method of blue native polyacrylamide gel electrophoresis and its combination with SDS-PAGE (BN/SDS-PAGE) were here employed to study the membrane complexome of an intracellular bacterium Francisella tularensis, the causative agent of a severe disease tularemia. In the presented study we describe the subunit composition and stoichiometry of several MPC involved in various cell functions (oxidative phosphorylation, membrane transport, cell division, membrane or periplasmic proteins folding, iron storage, phospholipid and cell envelope biosynthesis). Moreover, some undocumented or hypothetical MPC with possible connection to virulence factors were also proposed and some newly detected subunits were assigned to known complexes. The BN/SDS-PAGE combined with mass spectrometry appeared to be a strong tool in the investigation of membrane proteins and complexes and thus successfully complements the traditional electrophoresis approaches. The study of membrane proteins and membrane protein complexes (MPC) provides crucial information in the field of bacterial physiology and pathogenesis research. The method of blue native polyacrylamide gel electrophoresis and its combination with SDS-PAGE (BN/SDS-PAGE) were here employed to study the membrane complexome of an intracellular bacterium Francisella tularensis, the causative agent of a severe disease tularemia. In the presented study we describe the subunit composition and stoichiometry of several MPC involved in various cell functions (oxidative phosphorylation, membrane transport, cell division, membrane or periplasmic proteins folding, iron storage, phospholipid and cell envelope biosynthesis). Moreover, some undocumented or hypothetical MPC with possible connection to virulence factors were also proposed and some newly detected subunits were assigned to known complexes. The BN/SDS-PAGE combined with mass spectrometry appeared to be a strong tool in the investigation of membrane proteins and complexes and thus successfully complements the traditional electrophoresis approaches. (en)
Title	Francisella tularensis membrane complexome by blue native/SDS-PAGE Francisella tularensis membrane complexome by blue native/SDS-PAGE (en)
skos:prefLabel	Francisella tularensis membrane complexome by blue native/SDS-PAGE Francisella tularensis membrane complexome by blue native/SDS-PAGE (en)
skos:notation	RIV/60162694:G44__/11:00002451!RIV12-MO0-G44_____
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(OC 151), P(OVUOFVZ200808), S, Z(MO0FVZ0000501)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Dresler, Jiří Stulík, Jiří Klimentová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	200325
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/11:00002451
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Francisella tularensis; Tularemia; Blue native PAGE; Membrane protein complexes; Complexome (en)
http://linked.open.../riv/klicoveSlovo	Blue native PAGE Complexome Membrane protein complexes Tularemia Francisella tularensis
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[4685B4AEB1A7]
http://linked.open...i/riv/nazevZdroje	Journal of Proteomics
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20151 Development of new prophylactic tools against Francisella tularensis infection
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	75
http://linked.open...iv/tvurceVysledku	Dresler, Jiří Klimentová, Jana Stulík, Jiří
http://linked.open...ain/vavai/riv/wos	000298710400026
http://linked.open...n/vavai/riv/zamer	Zdravotnická problematika ZHN
issn	1874-3919
number of pages	13 (xsd:int)
http://localhost/t...ganizacniJednotka	G44
is http://linked.open...avai/riv/vysledek of	Francisella tularensis membrane complexome by blue native/SDS-PAGE

Faceted Search & Find service v1.16.116 as of Feb 22 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3239 as of Feb 22 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 68 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software