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| rdf:type
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| Description
| - An epitope extraction technique, based on biofunctionalized magnetic microspheres self-organized under a magnetic field in a channel of a simple microfluidic device fabricated from polydimethylsiloxane, was applied in the isolation and identification of prospective allergenic epitopes. Highly resistant ovalbumin was at first efficiently digested by a magnetic proteolytic reactor with trypsin and the second step, capture of allergenic epitopes from the mixture of peptides, was performed by a magnetic immunoaffinity carrier with orientedly immobilized rabbit anti-ovalbumin IgG molecules. Captured peptides were released with 0.05% trifluoroacetic acid. The elution fractions were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
- An epitope extraction technique, based on biofunctionalized magnetic microspheres self-organized under a magnetic field in a channel of a simple microfluidic device fabricated from polydimethylsiloxane, was applied in the isolation and identification of prospective allergenic epitopes. Highly resistant ovalbumin was at first efficiently digested by a magnetic proteolytic reactor with trypsin and the second step, capture of allergenic epitopes from the mixture of peptides, was performed by a magnetic immunoaffinity carrier with orientedly immobilized rabbit anti-ovalbumin IgG molecules. Captured peptides were released with 0.05% trifluoroacetic acid. The elution fractions were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. (en)
- Technika extrakce epitopů založená na bifunkčních magnetických mikrosférách samostatně organizovaných magnetickým polem v kanálku jednoduchého mikrofluidního čipu vyrobeného z polymethylsiloxanu byla aplikována na izolaci a identifikaci potenciálních alergenních epitopů. Vysoce rezistentní ovalbumin byl nejprve účinně štěpen trypsinem v magnetickém proteolytickém reaktoru a ve druhém kroku při použití magnetického imunoafinitního nosiče s orientovaně imobilizovanými králičími anti-ovalbuminovými IgG molekulami došlo k vazbě alergenních epitopů ze směsi peptidů. Vyvázané peptidy byly uvolněny 0,05% TFA. Eluční frakce byly analyzovány MALDI-TOF hmotnostním spektrometrem. (cs)
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| Title
| - Epitope mapping of allergen ovalbumin using biofunctionalized magnetic beads packed in microfluidic channels: The first step towards epitope-based vaccines
- Mapování alergenních epitopů ovalbuminu pomocí bifunkčních magnetických kuliček v mikrofluidních kanálcích: První krok k vakcínám založených na epitopech (cs)
- Epitope mapping of allergen ovalbumin using biofunctionalized magnetic beads packed in microfluidic channels: The first step towards epitope-based vaccines (en)
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| skos:prefLabel
| - Epitope mapping of allergen ovalbumin using biofunctionalized magnetic beads packed in microfluidic channels: The first step towards epitope-based vaccines
- Mapování alergenních epitopů ovalbuminu pomocí bifunkčních magnetických kuliček v mikrofluidních kanálcích: První krok k vakcínám založených na epitopech (cs)
- Epitope mapping of allergen ovalbumin using biofunctionalized magnetic beads packed in microfluidic channels: The first step towards epitope-based vaccines (en)
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| skos:notation
| - RIV/60162694:G44__/08:00001964!RIV09-MO0-G44_____
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(ME08105), S, Z(MO0FVZ0000604), Z(MSM0021627502)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60162694:G44__/08:00001964
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - allergen; epitope extraction; magnetic enzyme/immunoaffinity reactor; microfluidic device; epitope-based vaccine (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Chromatography A
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hernychová, Lenka
- Hubálek, Martin
- Jankovičová, B.
- Slováková, M.
- Viovy, J. L.
- Bilkova, Z.
- Rehulka, P.
- Rosnerová, S.
- Zverinova, Z.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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