About: New precedures of immunological diagnosis of potato viruses based on magnetic bead Technology

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Description	In this study the specificity and sensitivity of xMAP technology for multiplex detection of all six main potato viruses, with that of standard DAS ELISA were compared. Commercial diagnostic kits for ELISA as well as for Luminex xMAP (Prime Diagnostics, Wageningen, The Netherlands) were used. Signal intensity of ELISA (A405) and the same of MIA procedure, measured in calibrated AtheNA Luminex 200 Analyser (Biomedica) and expressed as MFI, were evaluated. Three times above the mean of healthy samples absorbance in ELISA and twice the fluorescence of virus-free controls in MIA were considered positive. The optimised concentration of individual antibodies, reagents, and MIA procedure, were adjusted in preliminary experiments with three isolates of each virus. Subsequently, using potato plantlets infected with particular PVY, PLRV, PVA, PVX and PVM isolates and maintained in in vitro. In this study the specificity and sensitivity of xMAP technology for multiplex detection of all six main potato viruses, with that of standard DAS ELISA were compared. Commercial diagnostic kits for ELISA as well as for Luminex xMAP (Prime Diagnostics, Wageningen, The Netherlands) were used. Signal intensity of ELISA (A405) and the same of MIA procedure, measured in calibrated AtheNA Luminex 200 Analyser (Biomedica) and expressed as MFI, were evaluated. Three times above the mean of healthy samples absorbance in ELISA and twice the fluorescence of virus-free controls in MIA were considered positive. The optimised concentration of individual antibodies, reagents, and MIA procedure, were adjusted in preliminary experiments with three isolates of each virus. Subsequently, using potato plantlets infected with particular PVY, PLRV, PVA, PVX and PVM isolates and maintained in in vitro. (en)
Title	New precedures of immunological diagnosis of potato viruses based on magnetic bead Technology New precedures of immunological diagnosis of potato viruses based on magnetic bead Technology (en)
skos:prefLabel	New precedures of immunological diagnosis of potato viruses based on magnetic bead Technology New precedures of immunological diagnosis of potato viruses based on magnetic bead Technology (en)
skos:notation	RIV/60109807:_____/12:#0000203!RIV13-MZE-60109807
http://linked.open...avai/predkladatel	Výzkumný ústav bramborářský Havlíčkův Brod, s.r.o.
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH71123)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Dědič, Petr
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav bramborářský Havlíčkův Brod, s.r.o.
http://linked.open...dnocenehoVysledku	154081
http://linked.open...ai/riv/idVysledku	RIV/60109807:_____/12:#0000203
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	microsphere immunoassay, Luminex xMAP, complexity (en)
http://linked.open.../riv/klicoveSlovo	complexity Luminex xMAP microsphere immunoassay
http://linked.open...ontrolniKodProRIV	[CD3E88E1A808]
http://linked.open...v/mistoKonaniAkce	Nitra
http://linked.open...i/riv/mistoVydani	Nitra
http://linked.open...i/riv/nazevZdroje	Zborník abstraktov z XIX. Slovenskej a českej konferencie o ochrane rastlín
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	Variability of Potato leafroll luteovirus (PLRV), improved reliability of its detection and utilization of transgenic resistance.
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Dědič, Petr
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2012-09-05 (xsd:date)
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Slovak University of Agriculture in Nitra, 2012, 1st edition
https://schema.org/isbn	978-80-552-0838-1
is http://linked.open...avai/riv/vysledek of	New precedures of immunological diagnosis of potato viruses based on magnetic bead Technology

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