| Attributes | Values |
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| rdf:type
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| Description
| - Metodou interference RNA (RNAi) jsme studovali funkci, stabilitu a vzájemné interakce vybraných jaderně-kodovaných podjednotek respiračních komplexů III a IV z procyklických stádií Trypanosoma brucei. Pomocí westernové analýzy byla v mitochondriálních lyzátech 4-6 dní po indukci RNAi prokázána úplná eliminace cílových proteinů. Naopak, jiná podjednotka komplexu IV zůstala při eliminaci cox6 neovlivněna. Jak bylo ukázáno prostřednictvím dvourozměrné nativní elektroforézy, způsobilo vypnutí jedné podjednotky rozpad celého příslušného komplexu, zatímco ostatní komplexy zůstaly intaktní. Ve všech knock-downech rovněž poklesl membránový potenciál a drasticky poklesly aktivity komplexů III a IV, ale ne komplexu I. Za pomoci specifických inhibitorů jsme ukázali, že procyklická stádia s poškozenými respiračními komplexy častečně přesměrují tok elektronů na alternativní oxidázu. Rovněž byla prokázána absence superkomplexu z komplexů I a III u T. brucei. (cs)
- The function, stability and mutual interactions of selected nuclear-encoded subunits of respiratory complexes III and IV were studied in the Trypanosoma brucei procyclics using RNA interference (RNAi). Western analysis of mitochondrial lysates using specific antibodies confirmed complete elimination of the targeted proteins 4 to 6 days post-induction. As revealed by two-dimensional blue native/SDS PAGE electrophoresis, silencing of a single subunit resulted in the disruption of the respective complex, while the other complex remained unaffected. Membrane potential was reproducibly decreased in the knock-downs and the activities of complex III and/or IV, but not complex I, were drastically reduced, as measured by activity assays and histochemical staining. The apparent absence in T. brucei procyclics of a supercomplex composed of complexes I and III may represent an ancestral state of the respiratory chain.
- The function, stability and mutual interactions of selected nuclear-encoded subunits of respiratory complexes III and IV were studied in the Trypanosoma brucei procyclics using RNA interference (RNAi). Western analysis of mitochondrial lysates using specific antibodies confirmed complete elimination of the targeted proteins 4 to 6 days post-induction. As revealed by two-dimensional blue native/SDS PAGE electrophoresis, silencing of a single subunit resulted in the disruption of the respective complex, while the other complex remained unaffected. Membrane potential was reproducibly decreased in the knock-downs and the activities of complex III and/or IV, but not complex I, were drastically reduced, as measured by activity assays and histochemical staining. The apparent absence in T. brucei procyclics of a supercomplex composed of complexes I and III may represent an ancestral state of the respiratory chain. (en)
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| Title
| - Downregulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei
- Eliminace jaderně kódovaných podjednotek komplexů III a IV způsobuje rozpad těchto komplexů ale ne komplexu I u Trypanosoma brucei (cs)
- Downregulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei (en)
|
| skos:prefLabel
| - Downregulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei
- Eliminace jaderně kódovaných podjednotek komplexů III a IV způsobuje rozpad těchto komplexů ale ne komplexu I u Trypanosoma brucei (cs)
- Downregulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei (en)
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| skos:notation
| - RIV/60077409:_____/05:00023757!RIV06-AV0-60077409
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(IAA5022302), Z(AV0Z60220518), Z(MSM6007665801)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60077409:_____/05:00023757
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - respiratory complex; Trypanosoma; RNA interference (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Horváth, A.
- Lukeš, Julius
- Verner, Zdeněk
- Horáková, Eva
- Cuninková, Ľ.
- Dunajčíková, P.
- Pravdová, E.
- Šlapetová, Iveta
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
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| is http://linked.open...avai/riv/vysledek
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