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| Description
| - Gut-content analyses using molecular techniques are an effective approach to quantifying predator-prey interactions. Predation is often assumed but scavenging is an equally likely route by which animal DNA enters the gut of a predator/scavenger. We used PCR (polymerase chain reaction) to detect scavenged material in predator gut homogenates. The rates at which DNA in decaying slugs ( Mollusca: Pulmonata) and aphids (Homoptera: Aphididae) became undetectable were estimated. The detectability of DNA from both carrion types in the guts of the generalist predator Pterostichus melanarius ( Coleoptera: Carabidae) was then determined. The effects of carrion age and weight, as well as beetle sex, on detection periods, were quantified. Laboratory trials measured prey preference of beetles between live and decaying prey. Further experiments measured, for the first time, feeding by P. melanarius on dead slugs and aphids directly in the field.
- Gut-content analyses using molecular techniques are an effective approach to quantifying predator-prey interactions. Predation is often assumed but scavenging is an equally likely route by which animal DNA enters the gut of a predator/scavenger. We used PCR (polymerase chain reaction) to detect scavenged material in predator gut homogenates. The rates at which DNA in decaying slugs ( Mollusca: Pulmonata) and aphids (Homoptera: Aphididae) became undetectable were estimated. The detectability of DNA from both carrion types in the guts of the generalist predator Pterostichus melanarius ( Coleoptera: Carabidae) was then determined. The effects of carrion age and weight, as well as beetle sex, on detection periods, were quantified. Laboratory trials measured prey preference of beetles between live and decaying prey. Further experiments measured, for the first time, feeding by P. melanarius on dead slugs and aphids directly in the field. (en)
- Analýzy střevních obsahů jsou stále častěji užívány pro detekci potravy predátorů. Problémem je, že DNA se ve střevech predátora může ocitnout i po konzumaci mrtvé kořisti. Pomocí PCR jsme sledovali, za jak dlouho ve střevech střevlíka Pterostichus melanarius přestanou být detekovatelní zkonzumovaní slimáci a mšice, laboratorně jsme měřili preferenci pro čerstvou a rozkládající se kořist, a konečně jsme v terénu měrili atraktivitu rozkládajících se kořisti. Střevlík v terénu i laboratoři preferoval rozloženou kořist, a PCR nebyla schopna rozlišit, zda pozřel kořist čerstvou nebo již rozloženou. Výsledky ukazují, že analýzy střevních obsahů jež neberou v potaz preferenci rozkládající se potravy mohou přeceňovat schopnosti střevlíků coby predátorů. (cs)
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| Title
| - The significance of facultative scavenging in generalist predator nutrition: detecting decayed prey in the guts of predators using PCR
- Fakultativní mrchožravost v potravě generalistického predátora: užití PCR k detekci rozkládající se kořisti v trávicím traktu predátora (cs)
- The significance of facultative scavenging in generalist predator nutrition: detecting decayed prey in the guts of predators using PCR (en)
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| skos:prefLabel
| - The significance of facultative scavenging in generalist predator nutrition: detecting decayed prey in the guts of predators using PCR
- Fakultativní mrchožravost v potravě generalistického predátora: užití PCR k detekci rozkládající se kořisti v trávicím traktu predátora (cs)
- The significance of facultative scavenging in generalist predator nutrition: detecting decayed prey in the guts of predators using PCR (en)
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| skos:notation
| - RIV/60077395:_____/05:00030116!RIV06-AV0-60077395
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077395:_____/05:00030116
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - aphid; DNA degradation; gut content (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Konvička, Martin
- Foltán, P.
- Sheppard, S.
- Symondson, W. O. C.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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