About: A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods

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About: A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Arthropod ecology and taxonomy have witnessed a substantial increase in the use of various trapping and molecular methods in the past two decades. However, the methods of collecting and the preservation liquids most widely used in arthropod traps do not properly preserve DNA. A stable and cheap substance that can be used to trap arthropods in and preserve their DNA is therefore needed. We test whether SDS/EDTA water solution can preserve DNA of beetles for one, four and eight weeks. Preservation of DNA was tested using PCR amplification of parts of the mitochondrial cytochrome c oxidase I (Cox1) and nuclear 28S rRN A genes. All the solutions tested preserved DNA for at least up to eight weeks and we recommend 2% SDS and 100mM EDTA as a cheap, stable and easily transportable alternative to ethanol for preserving specimens and their DNA collected in the field. This solution is also suitable for using as the collection and preservation liquid in arthropod traps. Arthropod ecology and taxonomy have witnessed a substantial increase in the use of various trapping and molecular methods in the past two decades. However, the methods of collecting and the preservation liquids most widely used in arthropod traps do not properly preserve DNA. A stable and cheap substance that can be used to trap arthropods in and preserve their DNA is therefore needed. We test whether SDS/EDTA water solution can preserve DNA of beetles for one, four and eight weeks. Preservation of DNA was tested using PCR amplification of parts of the mitochondrial cytochrome c oxidase I (Cox1) and nuclear 28S rRN A genes. All the solutions tested preserved DNA for at least up to eight weeks and we recommend 2% SDS and 100mM EDTA as a cheap, stable and easily transportable alternative to ethanol for preserving specimens and their DNA collected in the field. This solution is also suitable for using as the collection and preservation liquid in arthropod traps. (en)
Title	A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods (en)
skos:prefLabel	A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods (en)
skos:notation	RIV/60077344:_____/14:00427515!RIV15-GA0-60077344
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA204/09/1667), P(GA206/09/0115), P(GAP504/12/1952), P(TA02021501)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Čížek, Lukáš Pokluda, Pavel Drag, Lukáš Lukeš, Julius Novotný, Vojtěch Černotíková, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	756
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/14:00427515
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	insects; beetles; DNA preservation (en)
http://linked.open.../riv/klicoveSlovo	insects beetles DNA preservation
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[042B41C41B2F]
http://linked.open...i/riv/nazevZdroje	European Journal of Entomology
http://linked.open...in/vavai/riv/obor	EG
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Characterization of a novel protein complex involved in RNA processing and editing in the mitochondrion of Trypanosoma brucei Ecological determinants of biodiversity distribution in tropical forests: a synthesis for Lepidoptera communities in New Guinea Management of populations of insect species protected by EU: Effective, evidence based conservation of saproxylic beetles listed in the EU Habitats Directive Saproxylic diversity in space and time: From landscape history to community ecology and habitat modelling
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	111
http://linked.open...iv/tvurceVysledku	Lukeš, Julius Novotný, Vojtěch Drag, Lukáš Pokluda, Pavel Čížek, Lukáš Stříbrná, Eva
http://linked.open...ain/vavai/riv/wos	000343664100003
issn	1210-5759
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.14411/eje.2014.024

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