About: Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Bacillus thuringiensis Cry3A gene was redesigned for high expression in Norwegian spruce and the sequence was slightly modified to allow for simple N- and C- terminal deletions and domain II loop 1 exchange for synthetic oligos. Modified Cry3A toxins from 13 variants of the synthetic gene were expressed in Escherichia coli BL21 and their toxicity on spruce bark beetle larvae was tested using spruce bark sandwiches. Mutant toxins with N-terminal deletion and loop 1 duplication showed increased toxicity. Bacillus thuringiensis Cry3A gene was redesigned for high expression in Norwegian spruce and the sequence was slightly modified to allow for simple N- and C- terminal deletions and domain II loop 1 exchange for synthetic oligos. Modified Cry3A toxins from 13 variants of the synthetic gene were expressed in Escherichia coli BL21 and their toxicity on spruce bark beetle larvae was tested using spruce bark sandwiches. Mutant toxins with N-terminal deletion and loop 1 duplication showed increased toxicity. (en)
Title	Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop (en)
skos:prefLabel	Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop (en)
skos:notation	RIV/60077344:_____/12:00391203!RIV13-GA0-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GAP502/11/1471), P(QH71290), Z(AV0Z50510513)
http://linked.open...iv/cisloPeriodika	86
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Vlasák, Josef Bříza, Jindřich Pavingerová, Daniela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	129138
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/12:00391203
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Bacillus thuringiensis; Ips typographus; Picea abies (en)
http://linked.open.../riv/klicoveSlovo	Bacillus thuringiensis Ips typographus Picea abies
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[1F5BD3828B8A]
http://linked.open...i/riv/nazevZdroje	African Journal of Biotechnology
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of transgenic tissue lines of spruce (Picea abies) showing high toxicity towards bark beatle (Scolytidae) species. Study of mechanisms of insect pests resistance against Bacillus thuringiensis Cry toxins
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	11
http://linked.open...iv/tvurceVysledku	Malá, J. Pavingerová, Daniela Vlasák, Josef Bříza, Jindřich Knížek, M. Modlinger, R.
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
issn	1684-5315
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.5897/AJB11.3806
is http://linked.open...avai/riv/vysledek of	Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 116 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software