About: The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.)

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About: The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.) Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Metabolic engineering of the flavonoid pathway may be used for modifying nutritional, pharmaceutical and ornamental properties of plants. The PAP1 (AtMYB75) is a R2R3 MYB family member and stimulates the expression of flavonoid pathway genes in Arabidopsis thaliana . Previously, PAP1 was introduced into Humulus lupulus plants by Agrobacterium-mediated genetic transformation. Employing quantitative PCR, we show that each transgenic plant harbors one copy of PAP1. The expression of the structural genes CHS_H1, CHI, and F3'H, essential for the biosynthesis of flavonoids, was elevated in PAP1 transgenic plants compared to the wildtype hop. Chemometric methods were successfully used to discriminate between wildtype and transgenic plants expressing the transcription factor PAP1. Thus, transcription factor PAP1 activated the expression of these three genes in transgenic plants, opening the possibility for improving the content of pharmaceutically important secondary metabolites in hop. Metabolic engineering of the flavonoid pathway may be used for modifying nutritional, pharmaceutical and ornamental properties of plants. The PAP1 (AtMYB75) is a R2R3 MYB family member and stimulates the expression of flavonoid pathway genes in Arabidopsis thaliana . Previously, PAP1 was introduced into Humulus lupulus plants by Agrobacterium-mediated genetic transformation. Employing quantitative PCR, we show that each transgenic plant harbors one copy of PAP1. The expression of the structural genes CHS_H1, CHI, and F3'H, essential for the biosynthesis of flavonoids, was elevated in PAP1 transgenic plants compared to the wildtype hop. Chemometric methods were successfully used to discriminate between wildtype and transgenic plants expressing the transcription factor PAP1. Thus, transcription factor PAP1 activated the expression of these three genes in transgenic plants, opening the possibility for improving the content of pharmaceutically important secondary metabolites in hop. (en)
Title	The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.) The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.) (en)
skos:prefLabel	The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.) The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.) (en)
skos:notation	RIV/60077344:_____/12:00381542!RIV13-GA0-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GA521/08/0740), Z(AV0Z50510513)
http://linked.open...iv/cisloPeriodika	7-8
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Matoušek, Jaroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	174851
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/12:00381542
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	metabolic engineering; Humulus lupulus L.; transcription factors; flavonoid biosynthesis (en)
http://linked.open.../riv/klicoveSlovo	flavonoid biosynthesis Humulus lupulus L. metabolic engineering transcription factors
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[9876BC213619]
http://linked.open...i/riv/nazevZdroje	BrewingScience
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular analysis of transcription factors of hop (H. lupulus L.) in relation to lupulin biosynthesis
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	65
http://linked.open...iv/tvurceVysledku	Matoušek, Jaroslav Weber, G. Farag, M. A. Gatica-Arias, A. Häntzschel, K. R.
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
issn	1866-5195
number of pages	9 (xsd:int)
is http://linked.open...avai/riv/vysledek of	The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.)

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