About: Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In conifers and other plants with long reproductive cycles, transformed embryogenic calli can serve as a convenient source of plant material for the testing insecticidal or fungicidal transgene efficiency. In this report, transgenic embryogenic callus was obtained after transformation of somatic embryos of Norway spruce (Picea abies) by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation was successful only with suitable embryogenic line sensitive to Agrobacterium. From nine embryogenic lines tested only one gave transgenic callus In conifers and other plants with long reproductive cycles, transformed embryogenic calli can serve as a convenient source of plant material for the testing insecticidal or fungicidal transgene efficiency. In this report, transgenic embryogenic callus was obtained after transformation of somatic embryos of Norway spruce (Picea abies) by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation was successful only with suitable embryogenic line sensitive to Agrobacterium. From nine embryogenic lines tested only one gave transgenic callus (en)
Title	Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants (en)
skos:prefLabel	Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants (en)
skos:notation	RIV/60077344:_____/11:00370255!RIV12-AV0-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QH71290), Z(AV0Z50510513)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Vlasák, Josef Bříza, Jindřich Niedermeierová, Hana Pavingerová, Daniela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	231895
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/11:00370255
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Agrobacterium tumefaciens; genetic engineering; GUS activity; Picea abies (L.) Karst (en)
http://linked.open.../riv/klicoveSlovo	Agrobacterium tumefaciens GUS activity Picea abies (L.) Karst genetic engineering
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[F3931224E83E]
http://linked.open...i/riv/nazevZdroje	Journal of Forest Science
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of transgenic tissue lines of spruce (Picea abies) showing high toxicity towards bark beatle (Scolytidae) species.
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Pavingerová, Daniela Vlasák, Josef Bříza, Jindřich Niedermeierová, Hana
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
issn	1212-4834
number of pages	4 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants

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