About: Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Polymerase chain reaction (PCR) with subsequent restriction fragment length polymorphism (RFLP) analysis is often used for phytoplasma identification and classification. Although these techniques are very sensitive and specific, in some cases, nonspecific reactions, false positives and negatives results as well as unusual or illegible profiles after RFLP analyses, amplification of plant host´s DNA or other difficulties occurred. Experiences with suitability of positive and negative controls integration in PCR, evaluation of critical samples and other difficulties in phytoplasma PCR/RFLP identification are reported. Polymerase chain reaction (PCR) with subsequent restriction fragment length polymorphism (RFLP) analysis is often used for phytoplasma identification and classification. Although these techniques are very sensitive and specific, in some cases, nonspecific reactions, false positives and negatives results as well as unusual or illegible profiles after RFLP analyses, amplification of plant host´s DNA or other difficulties occurred. Experiences with suitability of positive and negative controls integration in PCR, evaluation of critical samples and other difficulties in phytoplasma PCR/RFLP identification are reported. (en)
Title	Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses (en)
skos:prefLabel	Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses (en)
skos:notation	RIV/60077344:_____/11:00367362!RIV12-MSM-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1QS500510558), P(OC09021), P(QH71145), Z(AV0Z50510513)
http://linked.open...iv/cisloPeriodika	Suppl.
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Fránová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	194600
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/11:00367362
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA extraction; PCR; primers; RFLP (en)
http://linked.open.../riv/klicoveSlovo	PCR DNA extraction primers RFLP
http://linked.open...odStatuVydavatele	IT - Italská republika
http://linked.open...ontrolniKodProRIV	[A6F36362BB14]
http://linked.open...i/riv/nazevZdroje	Bulletin of Insectology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of phytopathogenes occurrence and its genetic variants Study of apple proliferation and pear decline phytoplasmas in the Czech Republic. Diagnostics of viruses and phytoplasmas in the breeding material of red clover.
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	64
http://linked.open...iv/tvurceVysledku	Fránová, Jana
http://linked.open...ain/vavai/riv/wos	000296681900139
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
issn	1721-8861
number of pages	2 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses

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