About: Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Rep gene occurring in the majority of plasmids associated with phytoplasmas was selected as a target for real-time PCR together with single-copy Tuf gene from the phytoplasma genome. Rep-specific primers amplified products from host plants infected with phytoplasmas of groups 16SrI, -II, -III, -V, -X, and -XII. Relative concentrations of plasmids ranged depending on strain from 1-2 copies to several hundred copies per each phytoplasma studied. Primers targeting the rep gene are proposed for direct PCR detection of phytoplasma plasmids from experimental periwinkle host as well as from natural plant hosts. Rep gene occurring in the majority of plasmids associated with phytoplasmas was selected as a target for real-time PCR together with single-copy Tuf gene from the phytoplasma genome. Rep-specific primers amplified products from host plants infected with phytoplasmas of groups 16SrI, -II, -III, -V, -X, and -XII. Relative concentrations of plasmids ranged depending on strain from 1-2 copies to several hundred copies per each phytoplasma studied. Primers targeting the rep gene are proposed for direct PCR detection of phytoplasma plasmids from experimental periwinkle host as well as from natural plant hosts. (en)
Title	Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants (en)
skos:prefLabel	Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants (en)
skos:notation	RIV/60077344:_____/11:00367361!RIV12-AV0-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QH91224), Z(AV0Z50510513)
http://linked.open...iv/cisloPeriodika	Suppl.
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Petrzik, Karel Sarkisova, Tatiana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	236855
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/11:00367361
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Plasmid; universal primers; rep gene (en)
http://linked.open.../riv/klicoveSlovo	Plasmid rep gene universal primers
http://linked.open...odStatuVydavatele	IT - Italská republika
http://linked.open...ontrolniKodProRIV	[3A7FA24E8031]
http://linked.open...i/riv/nazevZdroje	Bulletin of Insectology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Research on methods for pathogen-free propagation material of currants
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	64
http://linked.open...iv/tvurceVysledku	Petrzik, Karel Sarkisova, Tatiana Čurnová, L.
http://linked.open...ain/vavai/riv/wos	000296681900010
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
issn	1721-8861
number of pages	2 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants

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