About: Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen

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About: Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The larva of the drosophilid fly, Chymomyza costata, is probably the most complex metazoan organism that can survive submergence in liquid nitrogen (-196°C) in a fully hydrated state. We examined the associations between the physiological and biochemical parameters of differently acclimated larvae and their freeze tolerance. Profiling of 61 different metabolites identified proline as a prominent compound whose concentration increased from 20 to 147 mM during diapause transition and subsequent cold acclimation. This study provides direct evidence for the essential role of proline in high freeze tolerance. Differential scanning calorimetry analysis suggested that high proline levels, in combination with a relatively low content of osmotically active water and freeze dehydration, increased the propensity of the remaining unfrozen water to undergo a glass-like transition (vitrification) and thus facilitated the prevention of cryoinjury. The larva of the drosophilid fly, Chymomyza costata, is probably the most complex metazoan organism that can survive submergence in liquid nitrogen (-196°C) in a fully hydrated state. We examined the associations between the physiological and biochemical parameters of differently acclimated larvae and their freeze tolerance. Profiling of 61 different metabolites identified proline as a prominent compound whose concentration increased from 20 to 147 mM during diapause transition and subsequent cold acclimation. This study provides direct evidence for the essential role of proline in high freeze tolerance. Differential scanning calorimetry analysis suggested that high proline levels, in combination with a relatively low content of osmotically active water and freeze dehydration, increased the propensity of the remaining unfrozen water to undergo a glass-like transition (vitrification) and thus facilitated the prevention of cryoinjury. (en)
Title	Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen (en)
skos:prefLabel	Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen (en)
skos:notation	RIV/60077344:_____/11:00362689!RIV12-AV0-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GA203/09/2014), P(GA206/07/0269), Z(AV0Z50070508)
http://linked.open...iv/cisloPeriodika	32
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Šimek, Petr Košťál, Vladimír Zahradníčková, Helena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	203214
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/11:00362689
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	insect freeze tolerance; cold hardiness; glass transition (en)
http://linked.open.../riv/klicoveSlovo	glass transition cold hardiness insect freeze tolerance
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[12BEED2E5AC6]
http://linked.open...i/riv/nazevZdroje	Proceedings of the National Academy of Sciences of the United States of America
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Cold-acclimation in insects: Biosynthesis of cryoprotectants and restructuring of biological membranes New approaches to alkylchloroformate-mediated sample preparation for parallel GC-MS and LC-MS for metabolite analysis in biofluids
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	108
http://linked.open...iv/tvurceVysledku	Košťál, Vladimír Šimek, Petr Zahradníčková, Helena
http://linked.open...ain/vavai/riv/wos	000293691400023
http://linked.open...n/vavai/riv/zamer	Study of the regulation of insect organism, dynamics of insect populations and function of insects in ecosystems
issn	0027-8424
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1073/pnas.1107060108
is http://linked.open...avai/riv/vysledek of	Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen

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