About: Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Results: Hemelipoglycoprotein from sheep tick Dermacentor marginatus plasma was purified in native state by immunoprecipitation and denatured using electroelution from SDS-PAGE separated plasma. The protein (290 kDa) contains two subunits with molecular weights 100 and 95 kDa. It is glycosylated by high-mannose and complex N-glycans HexNAc(2)Hex(9), HexNAc(2)Hex(10), HexNAc(4)Hex(7), and HexNAc(4)Hex(8). The purified protein is able to agglutinate red blood cells and has galactose-and mannose-binding specificity. Feeding does not increase its amounts in male plasma. The hemelipoglycoprotein was detected also in hemocytes, salivary glands, and gut. In salivary glands, the protein was present in both glycosylated and nonglycosylated forms. In hemolymph, it is present in complexes with putative fibrinogen-related proteins. This, together with its carbohydrate-binding activity, suggests its possible involvement in tick innate immunity. Results: Hemelipoglycoprotein from sheep tick Dermacentor marginatus plasma was purified in native state by immunoprecipitation and denatured using electroelution from SDS-PAGE separated plasma. The protein (290 kDa) contains two subunits with molecular weights 100 and 95 kDa. It is glycosylated by high-mannose and complex N-glycans HexNAc(2)Hex(9), HexNAc(2)Hex(10), HexNAc(4)Hex(7), and HexNAc(4)Hex(8). The purified protein is able to agglutinate red blood cells and has galactose-and mannose-binding specificity. Feeding does not increase its amounts in male plasma. The hemelipoglycoprotein was detected also in hemocytes, salivary glands, and gut. In salivary glands, the protein was present in both glycosylated and nonglycosylated forms. In hemolymph, it is present in complexes with putative fibrinogen-related proteins. This, together with its carbohydrate-binding activity, suggests its possible involvement in tick innate immunity. (en)
Title	Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization (en)
skos:prefLabel	Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization (en)
skos:notation	RIV/60077344:_____/11:00359405!RIV12-MSM-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(KJB600960906), P(LC06009), Z(AV0Z60220518), Z(MSM6007665801)
http://linked.open...iv/cisloPeriodika	January
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Grubhoffer, Libor Vancová, Marie Štěrba, Ján
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	201878
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/11:00359405
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ORNITHODOROS PARKERI; BOOPHILUS MICROPLUS; MASS-SPECTROMETRY; CATTLE TICK; SOFT TICK; DORIN M; PROTEINS; HEMOLYMPH; VITELLOGENIN; LIPOPROTEIN (en)
http://linked.open.../riv/klicoveSlovo	MASS-SPECTROMETRY HEMOLYMPH BOOPHILUS MICROPLUS CATTLE TICK DORIN M LIPOPROTEIN ORNITHODOROS PARKERI PROTEINS SOFT TICK VITELLOGENIN
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[E9041847BAF3]
http://linked.open...i/riv/nazevZdroje	Parasites Vectors
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Glycoprotein analysis of Ixodes ricinus salivary glands Centre for molecular ecology of vectors and pathogens
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	4
http://linked.open...iv/tvurceVysledku	Grubhoffer, Libor Vancová, Marie Dupejová, J. Štěrba, Ján
http://linked.open...ain/vavai/riv/wos	000286388600001
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni EKOLOGICKÉ, EVOLUČNÍ A EXPERIMENTÁLNĚ BIOLOGICKÉ PŘÍSTUPY KE STUDIU VZNIKU A VÝZNAMU BIODIVERZITY
issn	1756-3305
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1186/1756-3305-4-4
is http://linked.open...avai/riv/vysledek of	Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization

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