About: A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The 16S–23S ribosomal internal transcribed spacer (ITS1) is often used as a subspecies or strain-specific molecular marker for various kinds of bacteria. However, the presence of different copies of ITS1 within a single genome has been reported. Such mosaicism may influence correct typing of many bacteria and therefore knowledge about exact configuration of this region in a particular genome is essential. In order to screen the variability of ITS1 among and within Pseudomonas syringae genomes, an oligonucleotide microarray targeting different configurations of ITS1 was developed. The microarray revealed seven distinct variants in 13 pathovars tested and detected mosaicism within the genomes of P. syringae pv. coronafaciens, pisi, syringae and tabaci. In addition, the findings presented here challenge the using of rRNA analysis for pathovar and strain determination. The 16S–23S ribosomal internal transcribed spacer (ITS1) is often used as a subspecies or strain-specific molecular marker for various kinds of bacteria. However, the presence of different copies of ITS1 within a single genome has been reported. Such mosaicism may influence correct typing of many bacteria and therefore knowledge about exact configuration of this region in a particular genome is essential. In order to screen the variability of ITS1 among and within Pseudomonas syringae genomes, an oligonucleotide microarray targeting different configurations of ITS1 was developed. The microarray revealed seven distinct variants in 13 pathovars tested and detected mosaicism within the genomes of P. syringae pv. coronafaciens, pisi, syringae and tabaci. In addition, the findings presented here challenge the using of rRNA analysis for pathovar and strain determination. (en)
Title	A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae (en)
skos:prefLabel	A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae (en)
skos:notation	RIV/60077344:_____/10:00345684!RIV11-GA0-60077344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP522/07/P338), Z(AV0Z50510513)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Lenz, Ondřej Mráz, Ivan Beran, Pavel Fousek, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	244688
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/10:00345684
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	microarray; ITS1; mosaicism (en)
http://linked.open.../riv/klicoveSlovo	ITS1 microarray mosaicism
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[180CE4308988]
http://linked.open...i/riv/nazevZdroje	Journal of Microbiological Methods
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular analysis of complex genom of Pseudomonas syringae pathovars and design of DNA-chip for their parallel detection
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	82
http://linked.open...iv/tvurceVysledku	Beran, Pavel Fousek, Jan Lenz, Ondřej Mráz, Ivan
http://linked.open...ain/vavai/riv/wos	000279567700014
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
issn	0167-7012
number of pages	5 (xsd:int)
is http://linked.open...avai/riv/vysledek of	A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 75 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software