About: Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris'     Goto   Sponge   NotDistinct   Permalink

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  • Asparagus officinalis plants with severe fasciation of some spears were observed in southern Bohemia between 1998 and 2007. Nucleic acids extracted from these and asymptomatic plants were assayed with nested polymerase chain reaction (PCR) using the phytoplasma-specific universal ribosomal primers P1/P7 and R16F2n/R2. The restriction profiles obtained from digestion of the PCR products with five endonucleases (AluI, HhaI, KpnI, MseI and RsaI) were identical in all phytoplasmas infecting asparagus in the Czech Republic and indistinguishable from those of phytoplasmas in the aster yellows group (subgroup 16SrI-B). Sequence analysis of 1754 bp of the ribosomal operon indicated that the closest related phytoplasmas were those associated with epilobium phyllody and onion yellows. This is the first report of the natural occurrence of 'Candidatus Phytoplasma asteris' in A. officinalis.
  • Asparagus officinalis plants with severe fasciation of some spears were observed in southern Bohemia between 1998 and 2007. Nucleic acids extracted from these and asymptomatic plants were assayed with nested polymerase chain reaction (PCR) using the phytoplasma-specific universal ribosomal primers P1/P7 and R16F2n/R2. The restriction profiles obtained from digestion of the PCR products with five endonucleases (AluI, HhaI, KpnI, MseI and RsaI) were identical in all phytoplasmas infecting asparagus in the Czech Republic and indistinguishable from those of phytoplasmas in the aster yellows group (subgroup 16SrI-B). Sequence analysis of 1754 bp of the ribosomal operon indicated that the closest related phytoplasmas were those associated with epilobium phyllody and onion yellows. This is the first report of the natural occurrence of 'Candidatus Phytoplasma asteris' in A. officinalis. (en)
Title
  • Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris'
  • Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris' (en)
skos:prefLabel
  • Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris'
  • Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris' (en)
skos:notation
  • RIV/60077344:_____/10:00342115!RIV11-AV0-60077344
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1QS500510558), Z(AV0Z50510513)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 247825
http://linked.open...ai/riv/idVysledku
  • RIV/60077344:_____/10:00342115
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • 16S Ribosomal-RNA; mycoplasmalike organisms; gene-sequences (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [FDFCF2692FCE]
http://linked.open...i/riv/nazevZdroje
  • Journal of Phytopathology - Phytopathologische Zeitschrift
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 158
http://linked.open...iv/tvurceVysledku
  • Petrzik, Karel
  • Fránová, Jana
http://linked.open...ain/vavai/riv/wos
  • 000275396600016
http://linked.open...n/vavai/riv/zamer
issn
  • 0931-1785
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