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rdf:type
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Description
| - Kinetoplastids encode a single nuclear tryptophanyl tRNA that contains a CCA anticodon able to decode the UGG codons used in cytoplasmic protein synthesis but cannot decode the mitochondrial UGA codons. Following mitochondrial import, this problem is circumvented in Trypanosoma brucei by specifically editing the tRNA(Trp) anticodon to UCA, which can now decode the predominant mitochondrial UGA tryptophan codons. This tRNA also undergoes an unusual thiolation at position 33. In other organisms, tRNA thiolation is mediated by the cysteine desulfurase, Nfs1. We show that the mitochondria-targeted TbNfs, is essential for thiolation of both cytosolic and mitochondrial tRNAs. Furthermore, thiolation affects tRNA stability in the cytoplasm but more surprisingly acts as a negative determinant for the essential C to U editing in T. brucei. This provides a first line of evidence for mitochondrial C to U editing regulation in this system.
- Kinetoplastids encode a single nuclear tryptophanyl tRNA that contains a CCA anticodon able to decode the UGG codons used in cytoplasmic protein synthesis but cannot decode the mitochondrial UGA codons. Following mitochondrial import, this problem is circumvented in Trypanosoma brucei by specifically editing the tRNA(Trp) anticodon to UCA, which can now decode the predominant mitochondrial UGA tryptophan codons. This tRNA also undergoes an unusual thiolation at position 33. In other organisms, tRNA thiolation is mediated by the cysteine desulfurase, Nfs1. We show that the mitochondria-targeted TbNfs, is essential for thiolation of both cytosolic and mitochondrial tRNAs. Furthermore, thiolation affects tRNA stability in the cytoplasm but more surprisingly acts as a negative determinant for the essential C to U editing in T. brucei. This provides a first line of evidence for mitochondrial C to U editing regulation in this system. (en)
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Title
| - Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria
- Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria (en)
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skos:prefLabel
| - Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria
- Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria (en)
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skos:notation
| - RIV/60077344:_____/09:00334949!RIV10-MSM-60077344
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(2B06129), P(GA204/06/1558), P(LC07032), Z(AV0Z60220518)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/09:00334949
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - T. brucei; tRNA; 2-thiolation; Fe-S cluster; editing (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Biological Chemistry
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Alfonzo, J. D.
- Lukeš, Julius
- Paris, Zdeněk
- Long, Shaojun
- Poliak, Pavel
- RUBIO, M. A. T.
- Wohlgamuth-Benedum, J. M.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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