About: Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein

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About: Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	All mitochondrial tRNA genes are encoded in nucleus of T. brucei and thus all tRNAs have to be imported from the cytosol. We have investigated that silencing of the cysteine desulfurase, TbNfs, the key enzyme in tRNA thiolation (s2U) and Fe-S cluster formation in vivo, has no effect on tRNA partitioning. This is especially surprising in light of a recent report suggesting that in L. tropica the Rieske Fe-S protein is an essential component of the RNA import complex. We also show that down-regulation of the Rieske protein by RNA interference, similar to the TbNfs knockdowns, has no effect on import. The data presented here supports: s(2)U is not a negative determinant for tRNA import; the Rieske protein is not an essential component of the import machinery, and since the Rieske protein is essential for respiration and maintenance of inner mitochondrial membrane potential, neither process plays a critical role in tRNA import. All mitochondrial tRNA genes are encoded in nucleus of T. brucei and thus all tRNAs have to be imported from the cytosol. We have investigated that silencing of the cysteine desulfurase, TbNfs, the key enzyme in tRNA thiolation (s2U) and Fe-S cluster formation in vivo, has no effect on tRNA partitioning. This is especially surprising in light of a recent report suggesting that in L. tropica the Rieske Fe-S protein is an essential component of the RNA import complex. We also show that down-regulation of the Rieske protein by RNA interference, similar to the TbNfs knockdowns, has no effect on import. The data presented here supports: s(2)U is not a negative determinant for tRNA import; the Rieske protein is not an essential component of the import machinery, and since the Rieske protein is essential for respiration and maintenance of inner mitochondrial membrane potential, neither process plays a critical role in tRNA import. (en)
Title	Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein (en)
skos:prefLabel	Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein (en)
skos:notation	RIV/60077344:_____/09:00334944!RIV10-MSM-60077344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06129), P(GA204/06/1558), P(LC07032), Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Lukeš, Julius Paris, Zdeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	326541
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/09:00334944
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	T. brucei; tRNA import; 2-thiolation; RIC; Rieske; Fe-S cluster (en)
http://linked.open.../riv/klicoveSlovo	tRNA import 2-thiolation Fe-S cluster RIC Rieske T. brucei
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F207D0569782]
http://linked.open...i/riv/nazevZdroje	R N A
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Model organisms as a key to the understanding of functions of disease-related human genes Center for Functional Genetics Functional analysis of mitochondrial proteins in Trypanosoma brucei
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Alfonzo, J. D. Lukeš, Julius Paris, Zdeněk RUBIO, M. A. T.
http://linked.open...ain/vavai/riv/wos	000267142400017
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni
issn	1355-8382
number of pages	9 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein

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