About: The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic

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About: The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The presence of PPV in sweet cherry (Prunus avium) has been previously reported within an experimental collection of cherry genotypes in Holovousy, Czech Republic. In this paper, the modified procedure of RNA isolation was successfully applied - one cycle of differential ultracentrifugation was inserted before standard RNA isolation using TRI Reagent. Five PPV virus isolates were detected by RT-PCR by commonly used P1/P2 primers. The strain specific PCR and sequence analysis of NIb-CP-3'NCR region (1375 bp length) of PPV isolate 13/2 CZ identified that isolate as member of PPV-Rec group. Studies are in progress to confirm biological transmission of the isolate. The presence of PPV in sweet cherry (Prunus avium) has been previously reported within an experimental collection of cherry genotypes in Holovousy, Czech Republic. In this paper, the modified procedure of RNA isolation was successfully applied - one cycle of differential ultracentrifugation was inserted before standard RNA isolation using TRI Reagent. Five PPV virus isolates were detected by RT-PCR by commonly used P1/P2 primers. The strain specific PCR and sequence analysis of NIb-CP-3'NCR region (1375 bp length) of PPV isolate 13/2 CZ identified that isolate as member of PPV-Rec group. Studies are in progress to confirm biological transmission of the isolate. (en)
Title	The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic (en)
skos:prefLabel	The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic (en)
skos:notation	RIV/60077344:_____/08:00339342!RIV10-AV0-60077344
http://linked.open...avai/riv/aktivita	N Z
http://linked.open...avai/riv/aktivity	N, Z(AV0Z50510513)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Fránová, Jana
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	385959
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/08:00339342
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Plum pox virus; Prunus avium; sweet cherry (en)
http://linked.open.../riv/klicoveSlovo	sweet cherry Plum pox virus Prunus avium
http://linked.open...ontrolniKodProRIV	[55BA99AB15FF]
http://linked.open...v/mistoKonaniAkce	Antalya
http://linked.open...i/riv/mistoVydani	Leueven 1
http://linked.open...i/riv/nazevZdroje	Proceedings of the twentieth international symposium on virus and virus-liked diseases of temperature fruit crops-fruit tree diseases
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Fránová, Jana Kučerová, J. Gadiou, S. Navrátil, M. Šafářová, D. Talacko, L.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000256218900030
http://linked.open.../riv/zahajeniAkce	2006-05-22 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	ISHS Acta Horticulturea
https://schema.org/isbn	978-90-6605-080-8
is http://linked.open...avai/riv/vysledek of	The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic

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