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| rdf:type
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| Description
| - We compared quantitative polymerase chain reaction (Q-PCR) with two traditional methods, estimation in situ and direct counting with the aid of a hemocytometer, to evaluate their usefulness. Phlebotomus duboscqi and Ph. arabicus were infected with Leishmania major and L. infantum, respectively, and different approaches were compared to determine the intensity of Leishmania infections before and after defecation of the bloodmeal. Estimation of parasite numbers in situ is only a semiquantitative method, but it is quick and provides data about localization of infection. We recommend this technique for low-intensity infections after the bloodmeal is passed. Counting in a hemocytometer is a suitable technique for heavily infected sand flies or for quantification of Leishmania within the bloodmeal. The most accurate approach for parasite quantification in any type of sand fly infection is Q-PCR. This method is also highly sensitive and can detect one parasite per gut.
- We compared quantitative polymerase chain reaction (Q-PCR) with two traditional methods, estimation in situ and direct counting with the aid of a hemocytometer, to evaluate their usefulness. Phlebotomus duboscqi and Ph. arabicus were infected with Leishmania major and L. infantum, respectively, and different approaches were compared to determine the intensity of Leishmania infections before and after defecation of the bloodmeal. Estimation of parasite numbers in situ is only a semiquantitative method, but it is quick and provides data about localization of infection. We recommend this technique for low-intensity infections after the bloodmeal is passed. Counting in a hemocytometer is a suitable technique for heavily infected sand flies or for quantification of Leishmania within the bloodmeal. The most accurate approach for parasite quantification in any type of sand fly infection is Q-PCR. This method is also highly sensitive and can detect one parasite per gut. (en)
- V rámci této studie jsme porovnávali kvantitativní PCR (Q-PCR) s tradičními metodami k posouzení množství leishmánií v infikovaných vektorech (komárků rodu flebotomus), a to s odhadem infekce při pitvě flebotoma a s počítáním parazitů v počítací komůrce. Použili jsme flebotomy druhů Phlebotomus duboscqi a Ph. arabicus, kteří byli infikování Leishmania major a L. infantum. Stanovení počtu parazitů proběhlo před a po defekaci. Odhad při pitvě flebotoma je pouze semi-kvantitativní metoda, je však rychlá a umožňuje určit přesnou lokalizaci infekce. Stanovení počtu pomocí počítací komůrky je vhodné pouze u flebotomů s nižším počtem parazitů a pouze po defekaci. Jako nejpřesnější metoda se jeví použití Q-PCR, tato metoda je navíc velmi citlivá a dokáže stanovit I jediného parazita ve střevě flebotoma. (cs)
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| Title
| - Leishmania in sand flies: comparison of quantitative polymerase chain reaction with other techniques to determine the intensity of infection
- Leishmania ve flebotomech: srovnání kvantitativní PCR s ostatními technikami kvantifikace infekce (cs)
- Leishmania in sand flies: comparison of quantitative polymerase chain reaction with other techniques to determine the intensity of infection (en)
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| skos:prefLabel
| - Leishmania in sand flies: comparison of quantitative polymerase chain reaction with other techniques to determine the intensity of infection
- Leishmania ve flebotomech: srovnání kvantitativní PCR s ostatními technikami kvantifikace infekce (cs)
- Leishmania in sand flies: comparison of quantitative polymerase chain reaction with other techniques to determine the intensity of infection (en)
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| skos:notation
| - RIV/60077344:_____/08:00318321!RIV09-MSM-60077344
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(LC06009), Z(AV0Z60220518), Z(MSM0021620828)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/08:00318321
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - sand fly; Leishmania; Phlebotomus; parasite-vector interaction; RT-PCR (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Medical Entomology
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Volf, P.
- Votýpka, Jan
- Myšková, J.
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| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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