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| rdf:type
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| Description
| - Testovali jsme možnost nakažení larev klíšťat boreliemi ponořením do suspenze spirochét, metodu již dříve publikovanou, pro klíště Ixodes ricinus a tři nejvýznamnější evropské poddruhy borelií – B. burgdorferi s. s., B. garinii a B. afzelii. Tuto metodu jsme porovnávali s metodou infekce nymf klíšťat sáním na infikovaných myších. Oběmi metodami bylo dosaženo srovnatelných výsledků (44-65% míra infekce), s výjimkou B. afzelii, pro kterou bylo dosaženo lepších výsledků metodou ponoření klíšťat do suspenze (44%) ve srovnání se sáním na infikovaných myších (16%). Nymfy klíšťat infikovaných v larválním stádiu ponořením do suspenze borelií byly schopné přenést infekci do myší, což bylo prokázáno jak sérologií, tak i detekcí bakteriální DNA v orgánech myší pomocí PCR. Metoda ponořování je na rozdíl od sání na infekčních zvířatech rychlejší, umožňuje reprodukovatelnější podmínky infekce a může být proto využita pro studie jak přenosu patogenu, tak i interakce mezi boreliemi a vektorem. (cs)
- A previously reported procedure for the introduction of Borrelia spirochetes into tick larvae by immersion in a suspension of spirochetes was tested on Ixodes ricinus ticks and 3 of the most medically important European Borrelia genomic species, B. burgdorferi sensu stricto, B. garinii and B. afzelii. The procedure was compared with %22classical%22 infection of nymphs by feeding on infected mice. Both methods yielded comparable results (infection rate 44-65%) with the exception of B. afzelii, which produced better results using the immersion method (44%) compared with feeding on infected mice (16%). Nymphs infected by the immersion method at the larval stage were able to transmit the infection to naïve mice as shown by serology and PCR detection of spirochetal DNA in organs. The immersion method is faster than feeding on infected mice and provides more reproducible conditions for infection. It can be exploited for studies on both pathogen transmission and Borrelia-vector interactions.
- A previously reported procedure for the introduction of Borrelia spirochetes into tick larvae by immersion in a suspension of spirochetes was tested on Ixodes ricinus ticks and 3 of the most medically important European Borrelia genomic species, B. burgdorferi sensu stricto, B. garinii and B. afzelii. The procedure was compared with %22classical%22 infection of nymphs by feeding on infected mice. Both methods yielded comparable results (infection rate 44-65%) with the exception of B. afzelii, which produced better results using the immersion method (44%) compared with feeding on infected mice (16%). Nymphs infected by the immersion method at the larval stage were able to transmit the infection to naïve mice as shown by serology and PCR detection of spirochetal DNA in organs. The immersion method is faster than feeding on infected mice and provides more reproducible conditions for infection. It can be exploited for studies on both pathogen transmission and Borrelia-vector interactions. (en)
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| Title
| - Two ways of experimental infection of Ixodes ricinus ticks (Acari: Ixodidae) with spirochetes of Borrelia burgdorferi sensu lato complex
- Two ways of experimental infection of Ixodes ricinus ticks (Acari: Ixodidae) with spirochetes of Borrelia burgdorferi sensu lato complex (en)
- Dva způsoby experimentální infekce klíšťat Ixodes ricinus bakteriemi z komplexu Borrelia burgdorferi sensu lato (cs)
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| skos:prefLabel
| - Two ways of experimental infection of Ixodes ricinus ticks (Acari: Ixodidae) with spirochetes of Borrelia burgdorferi sensu lato complex
- Two ways of experimental infection of Ixodes ricinus ticks (Acari: Ixodidae) with spirochetes of Borrelia burgdorferi sensu lato complex (en)
- Dva způsoby experimentální infekce klíšťat Ixodes ricinus bakteriemi z komplexu Borrelia burgdorferi sensu lato (cs)
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| skos:notation
| - RIV/60077344:_____/08:00318072!RIV09-GA0-60077344
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA524/05/0811), P(LC06009), Z(AV0Z60220518), Z(MSM6007665801)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/08:00318072
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Lyme disease spirochete; Borrelia burgdorferi; Borrelia baronii; Borrelia afzelii; tick; Ixodes ricinus (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Fišerová, Lenka
- Kopecký, Jan
- Černá, Kateřina
- Horká, Helena
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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