About: Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Metodou PCR za použití primerů EHR521/747 bylo vyšetřeno na přítomnost Anaplasma phagocytophilum (Foggie, 1949) Dumler et al., 2001 v České republice 150 nymf klíšťat Ixodes ricinus (L., 1758) (Acarina: Ixodidae): 22 z 50 vyšetřených poolů bylo pozitivních (minimální prevalence 14,7 %). Naproti tomu však sekvenace PCR produktů neukázala úplnou shodu s A. phagocytophilum (91 %), nejvyšší nukleotidová shoda byla zjištěna s %22Candidatus Ehrlichia walkerii%22 (95 %). Výsledky naznačují potřebu vysoké obezřetnosti a opatrnosti při prezentaci PCR výsledků a nutnost optimalizace PCR metody pro detekci A. phagocytophilum v klíšťatech. (cs) A total of 150 nymphal Ixodes ricinus (L., 1758) (Acari: Ixodidae) from the Czech Republic were examined for Anaplasma phagocytophilum (Foggie, 1949) Dumler et al., 2001 by PCR using EHR521/747 primers: 22 of 50 pools were positive (minimum prevalence, 14.7%). However, sequencing of the PCR products did not show complete homology with A. phagocytophilum (91%) while the closest relationship (95%) was found to Candidatus Ehrlichia walkerii. The results indicate a need for care in interpretation of Anaplasma PCR results and for PCR optimization for detecting A. phagocytophilum in ticks. A total of 150 nymphal Ixodes ricinus (L., 1758) (Acari: Ixodidae) from the Czech Republic were examined for Anaplasma phagocytophilum (Foggie, 1949) Dumler et al., 2001 by PCR using EHR521/747 primers: 22 of 50 pools were positive (minimum prevalence, 14.7%). However, sequencing of the PCR products did not show complete homology with A. phagocytophilum (91%) while the closest relationship (95%) was found to Candidatus Ehrlichia walkerii. The results indicate a need for care in interpretation of Anaplasma PCR results and for PCR optimization for detecting A. phagocytophilum in ticks. (en)
Title	Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls Detekce DNA Anaplasma v klíšťatech Ixodes ricinus: nástrahy (cs) Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls (en)
skos:prefLabel	Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls Detekce DNA Anaplasma v klíšťatech Ixodes ricinus: nástrahy (cs) Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls (en)
skos:notation	RIV/60077344:_____/07:00096326!RIV08-AV0-60077344
http://linked.open.../vavai/riv/strany	310;312
http://linked.open...avai/riv/aktivita	P R V Z
http://linked.open...avai/riv/aktivity	P(GA206/03/0726), P(GA524/06/1479), P(KJB600930613), P(LC06009), R, V, Z(AV0Z60220518), Z(AV0Z60930519)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Grubhoffer, Libor Rudenko, Natalia Golovchenko, Maryna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	416511
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/07:00096326
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PCR detection; Ehrlichia; primer specificity (en)
http://linked.open.../riv/klicoveSlovo	Ehrlichia PCR detection primer specificity
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[869E40541E97]
http://linked.open...i/riv/nazevZdroje	Folia Parasitologica
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for molecular ecology of vectors and pathogens Comparative analysis of differential gene expression in Ixodes ricinus tick after infection with TBE virus and LB spirochetes Ecology of emerging arthropod-borne microorganisms Diversity of cultivable microorganisms of ixodid ticks, recognized vectors of vertebrate pathogens
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Golovchenko, Maryna Grubhoffer, Libor Rudenko, Natalia Hubálek, Zdeněk Rudolf, Ivo Šikutová, Silvie
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni Biodiverzita a ekologie obratlovců: Strategie ochrany a využívání přírodních populací
issn	0015-5683
number of pages	3 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls

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