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| rdf:type
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| Description
| - Většina motýlů má pohlavní chromosomy WZ/ZZ. Srovnávali jsme strukturu chromosomů W u Ephestia kuehniella, Cadra cautella, Plodia interpunctella a Galleria mellonella (Pyralidae). U každého druhu jsme laserovou mikrodisekcí W-chromatinu ze samičích polyploidních jader připravili malovací sondu, specifickou pro chromosom W. W-sondu z Ephestia jsme pak hybridizovali k chromosomům ostatních druhů, abychom detekovali homologní úseky jejich chromosomů W. Naopak druhově specifické W-sondy byly použity k identifikaci chromosomu W příslušného druhu. Tato Zoo-FISH odhalila částečnou homologii chromosomů W mezi E. kuehniella a dvěma dalšími druhy, C. cautella a P. interpunctella, a nepatrné zbytky homologie u G. mellonella. Komparativní genomové hybridizace ukázala, že chromosomy W jsou převážně tvořeny všudypřítomnou repetitivní DNA, ale obsahují také DNA specifickou pro samice. Naše výsledky podporují hypotézu o zrychlené molekulární divergenci chromosomů W při absenci meiotické rekombinace. (cs)
- Most Lepidoptera have a WZ/ZZ sex chromosome system. We compared structure of W chromosomes in Ephestia kuehniella, Cadra cautella, Plodia interpunctella, and Galleria mellonella (Pyralidae). In each species, we prepared a W-chromosome painting probe from laser-microdissected W-chromatin of female polyploid nuclei. The Ephestia W-probe was cross-hybridized to chromosomes of the other pyralids to detect common parts of their W chromosomes, while the species-specific W-probes identified the respective W chromosome. This Zoo-FISH revealed a partial homology of W-chromosome regions between E. kuehniella and two other pyralids, C. cautella and P. interpunctella, but almost no homology with G. mellonella. Comparative genomic hybridization revealed that the W chromosomes are composed mainly of ubiquitous repetitive DNA, but also possess female specific DNA. Our results support the hypothesis of the accelerated molecular divergence of the W chromosomes in the absence of meiotic recombination.
- Most Lepidoptera have a WZ/ZZ sex chromosome system. We compared structure of W chromosomes in Ephestia kuehniella, Cadra cautella, Plodia interpunctella, and Galleria mellonella (Pyralidae). In each species, we prepared a W-chromosome painting probe from laser-microdissected W-chromatin of female polyploid nuclei. The Ephestia W-probe was cross-hybridized to chromosomes of the other pyralids to detect common parts of their W chromosomes, while the species-specific W-probes identified the respective W chromosome. This Zoo-FISH revealed a partial homology of W-chromosome regions between E. kuehniella and two other pyralids, C. cautella and P. interpunctella, but almost no homology with G. mellonella. Comparative genomic hybridization revealed that the W chromosomes are composed mainly of ubiquitous repetitive DNA, but also possess female specific DNA. Our results support the hypothesis of the accelerated molecular divergence of the W chromosomes in the absence of meiotic recombination. (en)
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| Title
| - Molecular divergence of the W chromosomes in pyralid moths (Lepidoptera)
- Molecular divergence of the W chromosomes in pyralid moths (Lepidoptera) (en)
- Molekulární divergence chromosomů W u zavíječů (Lepidoptera) (cs)
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| skos:prefLabel
| - Molecular divergence of the W chromosomes in pyralid moths (Lepidoptera)
- Molecular divergence of the W chromosomes in pyralid moths (Lepidoptera) (en)
- Molekulární divergence chromosomů W u zavíječů (Lepidoptera) (cs)
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| skos:notation
| - RIV/60077344:_____/07:00090071!RIV08-AV0-60077344
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA206/06/1860), P(GD521/03/H160), V, Z(AV0Z50070508)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/07:00090071
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - CGH; chromosome painting; evolution (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Marec, František
- Vítková, Magda
- Kubíčková, S.
- Fuková, Iva
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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