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Description
  • Mitochondriální DNA prvoků skupiny Kinetoplastea je tvořena rozdílnými typy chromozómů - maxikroužky (nesou „normální“ geny) a velkým počtem minikroužků (kódují „guide RNA“ účastnící se RNA editingu). U trypanosom [Kinetoplastea] jsou DNA kroužky kondenzovány do jediného tělíska, kinetoplastu. Náš výzkum se zabývá otázkou, zda jsou multichromozomální mitochondriální genomy a kompaktní organizace chromozómů omezeny pouze na skupinu Kinetoplastea, nebo zda jsou rozšířeny v celé skupině Euglenozoa, tj. Kinetoplastea, Euglenida a Diplonemea. Pomocí světelné a elektronové mikroskopie a molekulárních technik byly zkoumány tyto organismy: Rhynchopus euleeides [Diplonemea] a Petalomonas cantuscygni, Peranema trichophorum a Entosiphon sulcatum [Euglenida]. Naše současná zjistění současně s dříve publikovanými výsledky ukazují, že multichromozomální mitochondriální genomy se vyskytují v celé skupině Euglenozoa, zatímco mitochondriální kinetoplastová DNA je typická pouze pro trypanozómy. (cs)
  • Mitochondrial DNA of Kinetoplastea is composed of different chromosomes, the maxicircle (bearing so-called regular genes) and numerous minicircles (specifying guide RNAs involved in RNA editing). In trypanosomes [Kinetoplastea], DNA circles are compacted into a single dense body, the kinetoplast. This report addresses the question whether multi-chromosome mitochondrial genomes and compacted chromosome organization are restricted to Kinetoplastea or rather occur throughout Euglenozoa, i.e., Kinetoplastea, Euglenida and Diplonemea. To this end, we investigated the diplonemid Rhynchopus euleeides and the euglenids Petalomonas cantuscygni, Peranema trichophorum and Entosiphon sulcatum, using light and electron microscopy and molecular techniques. Our findings together with previously published data show that multi-chromosome mitochondrial genomes prevail across Euglenozoa, while kinetoplast-like mtDNA packaging is confined to trypanosomes.
  • Mitochondrial DNA of Kinetoplastea is composed of different chromosomes, the maxicircle (bearing so-called regular genes) and numerous minicircles (specifying guide RNAs involved in RNA editing). In trypanosomes [Kinetoplastea], DNA circles are compacted into a single dense body, the kinetoplast. This report addresses the question whether multi-chromosome mitochondrial genomes and compacted chromosome organization are restricted to Kinetoplastea or rather occur throughout Euglenozoa, i.e., Kinetoplastea, Euglenida and Diplonemea. To this end, we investigated the diplonemid Rhynchopus euleeides and the euglenids Petalomonas cantuscygni, Peranema trichophorum and Entosiphon sulcatum, using light and electron microscopy and molecular techniques. Our findings together with previously published data show that multi-chromosome mitochondrial genomes prevail across Euglenozoa, while kinetoplast-like mtDNA packaging is confined to trypanosomes. (en)
Title
  • Unusual mitochondrial genome structures throughout the Euglenozoa
  • Neobvyklá struktura mitochondriálního genomu ve skupině Euglenozoa (cs)
  • Unusual mitochondrial genome structures throughout the Euglenozoa (en)
skos:prefLabel
  • Unusual mitochondrial genome structures throughout the Euglenozoa
  • Neobvyklá struktura mitochondriálního genomu ve skupině Euglenozoa (cs)
  • Unusual mitochondrial genome structures throughout the Euglenozoa (en)
skos:notation
  • RIV/60077344:_____/07:00087487!RIV08-AV0-60077344
http://linked.open.../vavai/riv/strany
  • 385;396
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(2B06129), P(GA204/06/1558), Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 456702
http://linked.open...ai/riv/idVysledku
  • RIV/60077344:_____/07:00087487
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • euglenozoan protists; mitochondrial chromosomes; mitochondrial ultrastructure (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [4CFFBC9670A5]
http://linked.open...i/riv/nazevZdroje
  • Protist
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 158
http://linked.open...iv/tvurceVysledku
  • Lukeš, Julius
  • Burger, G.
  • Faktorová, Drahomíra
  • Roy, J.
http://linked.open...n/vavai/riv/zamer
issn
  • 1434-4610
number of pages
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