| Attributes | Values |
|---|
| rdf:type
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| Description
| - Zaklonovali jsme, osekvenovali a exprimovali novou asparaginylovou endopeptidázu (legumain) z klíštěte Ixodes ricinus (IrAE), která je prvním reprezentantem klanu CD, rodiny C13 cysteinových peptidáz popsaným u členovců. IrAE byl exprimován výhradně v klíštěcích střevech a byl lokalizován v trávicích vesikulech a v peritrofické membráně obklopující trávicí buňky. Aktivní IrAE byl získán expresí v kvasinkách Pichia pastoris a jeho enzymatické vlasnosti byly charakterizovány. Za použití kombinatoriálních peptidických knihoven jsme potvrdili jeho striktní hydrolytickou specificitu pro asparagin v poloze P1. Diskutovali jsme možnou úlohu IrAE v trávicím systému klíštěte a porovnali jsme ji s trávením krve u krevních motolic. (cs)
- We have cloned, sequenced and expressed a novel asparaginyl endopeptidase (legumain) from the hard tick Ixodes ricinus (IrAE) which is the first representative of a clan CD family C13 cysteine protease described in arthropods. The IrAE is solely expressed in the tick gut and was localized in the digestive vesicles and within the perithrophic matrix surrounding the digestive cells. The active IrAE was expressed in the yeast Pichia pastoris and the enzyme properties were characterized. The use of combinatorial peptide libary approved the strict specificity of IrAE for asparagine residue at P1 position. The possible role of IrAE in tick blood digestion was discussed and compared to the digestive system of blood-flukes.
- We have cloned, sequenced and expressed a novel asparaginyl endopeptidase (legumain) from the hard tick Ixodes ricinus (IrAE) which is the first representative of a clan CD family C13 cysteine protease described in arthropods. The IrAE is solely expressed in the tick gut and was localized in the digestive vesicles and within the perithrophic matrix surrounding the digestive cells. The active IrAE was expressed in the yeast Pichia pastoris and the enzyme properties were characterized. The use of combinatorial peptide libary approved the strict specificity of IrAE for asparagine residue at P1 position. The possible role of IrAE in tick blood digestion was discussed and compared to the digestive system of blood-flukes. (en)
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| Title
| - IrAE - An asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus
- IrAE – asparaginylová endopeptidáza (legumain) ve střevě klíštěte Ixodes ricinus (cs)
- IrAE - An asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus (en)
|
| skos:prefLabel
| - IrAE - An asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus
- IrAE – asparaginylová endopeptidáza (legumain) ve střevě klíštěte Ixodes ricinus (cs)
- IrAE - An asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus (en)
|
| skos:notation
| - RIV/60077344:_____/07:00087229!RIV08-AV0-60077344
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA206/06/0865), P(GD524/03/H133), P(IAA6022307), P(LC06009), V, Z(AV0Z60220518)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/07:00087229
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - asparaginyl endopeptidase; tick gut,; blood digestion (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - AU - Australské společenství
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - International Journal for Parasitology
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Dvořák, J.
- Kopáček, Petr
- Vancová, Marie
- Sojka, Daniel
- Burešová, Veronika
- Franta, Zdeněk
- Hajdušek, Ondřej
- Caffrey, C. R.
- Craik, C. S.
- McKerrow, J. H.
- Sajid, M.
- Bogyo, M.
- Schneider, E. L.
- Sexton, K. B.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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