| Attributes | Values |
|---|
| rdf:type
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| Description
| - Virus klíšťové encefalitidy, „emerging“ patogen v řadě evropských a asijských zemí, je taxonomicky členěn na tři subtypy (evropský, sibiřský a dálněvýchodní). Zástupci jednotlivých subtypů způsobují onemocnění o různé závažnosti. Z toho důvodu je včasné určení subtypu v klinickém vzorku nebo v klíštěti vyjmutém z pacienta velmi významné. Zde prezentujeme metodu multiplex RT-PCR pro rychlou a jednoduchou subtypizaci izolátů viru klíšťové encefalitidy. Metoda je založena na unikátní kombinaci oligonukleotidových primerů, které hybridizují se pro subtyp konzervovanými sekvencemi virového obalového proteinu. Multiplexová RT-PCR najde své uplatnění také v molekulárně-epidemiologických studiích viru klíšťové encefalitidy. (cs)
- Tick-borne encephalitis virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of tick-borne encephalitis virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific signature positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of tick-borne encephalitis virus.
- Tick-borne encephalitis virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of tick-borne encephalitis virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific signature positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of tick-borne encephalitis virus. (en)
|
| Title
| - Rapid subtyping of tick-borne encephalitis virus isolates using multiplex RT-PCR
- Rapid subtyping of tick-borne encephalitis virus isolates using multiplex RT-PCR (en)
- Subtypová charakterizace izolátů viru klíšťové encefalitidy metodou multiplexové RT-PCR (cs)
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| skos:prefLabel
| - Rapid subtyping of tick-borne encephalitis virus isolates using multiplex RT-PCR
- Rapid subtyping of tick-borne encephalitis virus isolates using multiplex RT-PCR (en)
- Subtypová charakterizace izolátů viru klíšťové encefalitidy metodou multiplexové RT-PCR (cs)
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| skos:notation
| - RIV/60077344:_____/07:00087201!RIV08-AV0-60077344
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA524/06/1479), P(LC06009), S, Z(AV0Z60220518), Z(MSM6007665801)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/07:00087201
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - tick-borne encephalitis virus; subtyping; multiplex RT-PCR (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Virological Methods
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Grubhoffer, Libor
- Kopecký, Jan
- Růžek, Daniel
- Golovljova, I.
- Šťastná, B.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
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| is http://linked.open...avai/riv/vysledek
of | |
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