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| rdf:type
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| Description
| - Nový druh trypanosomatida, Leptomonas podlipaevi n. sp., je zde popsán na základě světelné mikroskopie, ultrastruktury a molekulárně fylogenetických dat. Tento organismus je jak v hostiteli, tak v kultuře pleomorfní, se dvěmi převládajícími formami – typicky promastigotní, s dlouhým bičíkem a kratší promastigotní s malým až sotva vysunutým bičíkem. Několik opakovaných úseků sekvencí pro úvodní sestřih RNA získaných z původních kultur a klonálních linií reprezentujících oba typy buněk byly téměř identické. Tyto sekvence vytvořily ve stromě konstruovaném metodou „neighbor-joining“ těsný kluster, který byl dobře oddělený od ostatních druhů trypanosomatid. Sekvence genu pro glyceraldehyd fosfát dehydrogenázu byly určeny pro L. podlipaevi a 10 v minulosti popsaných druhů trypanosomatidů. Molekulárně-fylogenetická analýza prokázala, že nový druh je nejblíže příbuzný druhům Leptomonas seymouri a Leptomonas pyrrhocoris. Analýza rovněž zvýraznila polyfylii rodu Leptomonas. (cs)
- Leptomonas podlipaevi n. sp., a new trypanosomatid species, is described herein based on light microscopic, ultrastructural, and molecular phylogenetic data. The organism is pleomorphic both in host and culture, with two predominant forms-a typical promastigote with a long flagellum and a shorter promastigote with a small or barely extending flagellum. Several spliced leader RNA repeat sequences obtained from the original cultures and the clonal lines representing two types of cells were all nearly identical. These sequences formed a tight cluster in the neighbor-joining tree well separated from other trypanosomatid species. Glyceraldehyde phosphate dehydrogenase gene sequences were determined for L. podlipaevi and 10 previously described trypanosomatid species. Molecular phylogenetic analysis has demonstrated that the new species is most closely related to Leptomonas seymouri and Leptomonas pyrrhocoris. The analysis has also highlighted the polyphyly of the genus Leptomonas.
- Leptomonas podlipaevi n. sp., a new trypanosomatid species, is described herein based on light microscopic, ultrastructural, and molecular phylogenetic data. The organism is pleomorphic both in host and culture, with two predominant forms-a typical promastigote with a long flagellum and a shorter promastigote with a small or barely extending flagellum. Several spliced leader RNA repeat sequences obtained from the original cultures and the clonal lines representing two types of cells were all nearly identical. These sequences formed a tight cluster in the neighbor-joining tree well separated from other trypanosomatid species. Glyceraldehyde phosphate dehydrogenase gene sequences were determined for L. podlipaevi and 10 previously described trypanosomatid species. Molecular phylogenetic analysis has demonstrated that the new species is most closely related to Leptomonas seymouri and Leptomonas pyrrhocoris. The analysis has also highlighted the polyphyly of the genus Leptomonas. (en)
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| Title
| - An integrated morphological and molecular approach to a new species description in the Trypanosomatidae: the case of Leptomonas podlipaevi n.sp., a parasite of Boisea rubrolineata (Hemiptera: Rhopalidae)
- An integrated morphological and molecular approach to a new species description in the Trypanosomatidae: the case of Leptomonas podlipaevi n.sp., a parasite of Boisea rubrolineata (Hemiptera: Rhopalidae) (en)
- Integrovaný morfologický a molekulární přístup k popisu nového druhu čeledi Trypanosomatidae: případ Leptomonas podlipaevi n. sp., parazita Boisea rubrolineata (Hemiptera: Rhopalidae) (cs)
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| skos:prefLabel
| - An integrated morphological and molecular approach to a new species description in the Trypanosomatidae: the case of Leptomonas podlipaevi n.sp., a parasite of Boisea rubrolineata (Hemiptera: Rhopalidae)
- An integrated morphological and molecular approach to a new species description in the Trypanosomatidae: the case of Leptomonas podlipaevi n.sp., a parasite of Boisea rubrolineata (Hemiptera: Rhopalidae) (en)
- Integrovaný morfologický a molekulární přístup k popisu nového druhu čeledi Trypanosomatidae: případ Leptomonas podlipaevi n. sp., parazita Boisea rubrolineata (Hemiptera: Rhopalidae) (cs)
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| skos:notation
| - RIV/60077344:_____/06:00048736!RIV07-AV0-60077344
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - Z(AV0Z60220518), Z(MSM6007665801)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/06:00048736
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Kinetoplastida; species description; phylogeny (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Eukaryotic Microbiology
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Lukeš, Julius
- Maslov, D. A.
- Yurchenko, V.
- Xu, X.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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