| Attributes | Values |
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| rdf:type
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| Description
| - Abychom prozkoumali vliv sekvenčního kontextu translačního iniciačního kodonu na efektivitu translace u Kinetoplastid, zkonstruovali jsme knihovnu expresních plazmidů s permutovanými třemi nukleotidy předcházejícími ATG reportérového genu kódujícího zlepšený zelený fluorescenční protein (EGFP). Úrovně exprese byly kvantifikovány přímo, měřením fluorescence živých buněk, a potvrzeny Westernovým přenosem. Sledovali jsme silný vliv pre-ATG tripletu na úroveň exprese. Abychom ozřejmili stupeň evoluční konzervace sledovaného efektu, transformovali jsme stejnou sadou konstruktů Phytomonas serpens, parazita rostlin náležejícího k trypanosomatidům. Translační efektivita zprostředkovaná jednotlivými pre-ATG triplety byla u tohoto druhu podobná jako u Leishmania tarentolae. Naproti tomu translační efektivita u dvou jiných proteinů, které obsahovaly vybrané pre-ATG triplety, nekorelovala ani s EGFP ani mezi těmito proteiny navzájem. (cs)
- To investigate the influence of sequence context of translation initiation codon on translation efficiency in Kinetoplastida, we constructed a library of expression plasmids randomized in the three nucleotides prefacing ATG of a reporter gene encoding enhanced green fluorescent protein (EGFP). The expression levels were quantified by measuring the fluorescence in living cells and confirmed by Western blotting. We observed a strong influence of the pre-ATG triplet on the level of protein expression. To understand the degree of evolutionary conservation of the observed effect, we transformed Phytomonas serpens, a trypanosomatid parasite of plants, with a subset of the constructs. The pattern of translational efficiency mediated by individual pre-ATG triplets in this species was similar to that observed in Leishmania tarentolae. However, the pattern of translational efficiency of two other proteins containing selected pre-ATG triplets did not correlate with either EGFP or each other.
- To investigate the influence of sequence context of translation initiation codon on translation efficiency in Kinetoplastida, we constructed a library of expression plasmids randomized in the three nucleotides prefacing ATG of a reporter gene encoding enhanced green fluorescent protein (EGFP). The expression levels were quantified by measuring the fluorescence in living cells and confirmed by Western blotting. We observed a strong influence of the pre-ATG triplet on the level of protein expression. To understand the degree of evolutionary conservation of the observed effect, we transformed Phytomonas serpens, a trypanosomatid parasite of plants, with a subset of the constructs. The pattern of translational efficiency mediated by individual pre-ATG triplets in this species was similar to that observed in Leishmania tarentolae. However, the pattern of translational efficiency of two other proteins containing selected pre-ATG triplets did not correlate with either EGFP or each other. (en)
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| Title
| - Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5´ sequence context
- Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5´ sequence context (en)
- Iniciace translace u Leishmania tarentolae a Phytomonas serpens (Kinetoplastida) je silně ovlivněna pre-ATG tripletem a jeho 5´ sekvenčním kontextem (cs)
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| skos:prefLabel
| - Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5´ sequence context
- Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5´ sequence context (en)
- Iniciace translace u Leishmania tarentolae a Phytomonas serpens (Kinetoplastida) je silně ovlivněna pre-ATG tripletem a jeho 5´ sekvenčním kontextem (cs)
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| skos:notation
| - RIV/60077344:_____/06:00048430!RIV07-AV0-60077344
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(1QS600220554), Z(AV0Z60220518), Z(MSM6007665801)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/06:00048430
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Kinetoplastida; translation; initiation (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Molecular and Biochemical Parasitology
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Jirků, Milan
- Lukeš, Julius
- Paris, Zdeněk
- Breitling, R.
- Alexandrov, K. A.
- Kushnir, S.
- Mureev, S.
- Pyatkov, K.
- Regmi, Sandesh
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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