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| Description
| - The enzyme DhaA from Rhodococcus rhodochrous NCIMB 13064 belongs to the haloalkane dehalogenases, which catalyze the hydrolysis of haloalkanes to the corresponding alcohols. The haloalkane dehalogenase DhaA and its variants can be used to detoxify the industrial pollutant 1,2,3-trichloropropane (TCP). Three mutants named DhaA04, DhaA14 and DhaA15 were constructed in order to study the importance of tunnels connecting the buried active site with the surrounding solvent to the enzymatic activity. All protein mutants were crystallized using the sitting-drop vapour-diffusion method. The crystals of DhaA04 belonged to the orthorhombic space group P2(1)2(1)2(1), while the crystals of the other two mutants DhaA14 and DhaA15 belonged to the triclinic space group P1. Native data sets were collected for the DhaA04, DhaA14 and DhaA15 mutants at beamline X11 of EMBL, DESY, Hamburg to the high resolutions of 1.30, 0.95 and 1.15 angstrom, respectively.
- The enzyme DhaA from Rhodococcus rhodochrous NCIMB 13064 belongs to the haloalkane dehalogenases, which catalyze the hydrolysis of haloalkanes to the corresponding alcohols. The haloalkane dehalogenase DhaA and its variants can be used to detoxify the industrial pollutant 1,2,3-trichloropropane (TCP). Three mutants named DhaA04, DhaA14 and DhaA15 were constructed in order to study the importance of tunnels connecting the buried active site with the surrounding solvent to the enzymatic activity. All protein mutants were crystallized using the sitting-drop vapour-diffusion method. The crystals of DhaA04 belonged to the orthorhombic space group P2(1)2(1)2(1), while the crystals of the other two mutants DhaA14 and DhaA15 belonged to the triclinic space group P1. Native data sets were collected for the DhaA04, DhaA14 and DhaA15 mutants at beamline X11 of EMBL, DESY, Hamburg to the high resolutions of 1.30, 0.95 and 1.15 angstrom, respectively. (en)
- Enzym DhaA z Rhodococcus rhodochrous NCIMB 13064 patří do skupiny haloalkan dehalogenas, které katalyzují hydrolýzu halogenovaných alkanů na příslušné alkoholy. Haloalkan dehalogenasa DhaA a její mutantní varianty můžou být použity k detoxikaci průmyslových polutantů, např. 1,2,3-trichloropropanu (TCP). Tři mutanty DhaA04, DhaA14 a DhaA15 byly připraveny ke studiu významu tunelů spojujících aktivní místo s okolním solventem. Všechny mutanty byly krystalizovány metodou difůze par v sedící kapce. Krystaly DhaA04 krystalizují v rombické prostorové grupě P212121 zatímco krystaly zbylých dvou mutantů DhaA14 a DhaA15 v triklinické P1. Difrakční data byla naměřena na synchrotronu DESY/EMBL (Hamburk, Německo) do vysokých rozlišení 1.30 Ǻ, 0.95 Å a 1.15 (cs)
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| Title
| - Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis
- Krystaly mutantů DhaA z Rhodococcus rhodochrous NCIMB 13064 difraktující do ultra vysokého rozlišení: krystalizace a předběžná difrakční analýza (cs)
- Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis (en)
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| skos:prefLabel
| - Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis
- Krystaly mutantů DhaA z Rhodococcus rhodochrous NCIMB 13064 difraktující do ultra vysokého rozlišení: krystalizace a předběžná difrakční analýza (cs)
- Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis (en)
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| skos:notation
| - RIV/60076658:12640/08:00009369!RIV09-MSM-12640___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(LC06010), S, Z(MSM6007665808)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12640/08:00009369
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - haloalkane dehalogenase gene; heterologous expression; 1,2,3-trichloropropane; 1,2-dichloroethane; refinement; evolution; enzyme (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Acta Crystallographica Section F-Structural Biology and Crystallization Communications
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Damborsky, Jiří
- Kutá-Smatanová, Ivana
- Stsiapanava, Alena
- Lapkouski, Mikalai
- Koudelakova, Tana
- Pavlova, Marina
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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