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Description
  • Role phosphatidylglycerolu (PG) ve fotosyntetických membránách sinic jsme studovali v mutantu sinice Synechocystis sp. PCC6803 s chybějícími fykobilisomy a s inaktivovaným genem cdsA kódujícím cytidine 5'-diphosphate diacylglycerol synthasu, klíčový enzym biosyntézy PG. PAL/Delta cdsA mutant rostl fotoautototrofně v médiu s dodaným PG, ale odstranění PG vedlo k: (a) zastavení růstu a dělení buněk, (b) k zpomalení elektronového transportu mezi akceptory QA a QB ve fotosystému II (PSII) a (c) modifikaci chlorofylových fluorescenčních křivek. Dvourozměrná elektroforéza ukázala, že absence PG vede (a) k rozpadu dimerní formy PSII na monomery, a (b) k odtržení CP43 od zbylé části komplexu PSII (cs)
  • To analyze the role of phosphatidylglycerol (PG) in photosynthetic membranes of cyanobacteria we used two mutants of Synechocystis sp. PCC6803: the PAL mutant which has no phycobilisomes and shows a high PSII/PSI ratio, and a mutant derived from it by inactivating its cdsA gene encoding cytidine 5'-diphosphate diacylglycerol synthase, a key enzyme in PG synthesis. In a medium supplemented with PG the PAL/Delta cdsA mutant cells grew photoautotrophically. Depletion of PG in the medium resulted (a) in an arrest of cell growth and division, (b) in a slowdown of electron transfer from the acceptor Q(A) to Q(B) in PSII and (c) in a modification of chlorophyll fluorescence curve. The depletion of PG affected neither the redox levels of QA nor the S-2 state of the oxygen-evolving manganese complex, as indicated by thermoluminescence studies. Two-dimensional PAGE showed that in the absence of PG (a) the PSII dimer was decomposed into monomers, and (b) the CP43 protein was detached from a major Part of th
  • To analyze the role of phosphatidylglycerol (PG) in photosynthetic membranes of cyanobacteria we used two mutants of Synechocystis sp. PCC6803: the PAL mutant which has no phycobilisomes and shows a high PSII/PSI ratio, and a mutant derived from it by inactivating its cdsA gene encoding cytidine 5'-diphosphate diacylglycerol synthase, a key enzyme in PG synthesis. In a medium supplemented with PG the PAL/Delta cdsA mutant cells grew photoautotrophically. Depletion of PG in the medium resulted (a) in an arrest of cell growth and division, (b) in a slowdown of electron transfer from the acceptor Q(A) to Q(B) in PSII and (c) in a modification of chlorophyll fluorescence curve. The depletion of PG affected neither the redox levels of QA nor the S-2 state of the oxygen-evolving manganese complex, as indicated by thermoluminescence studies. Two-dimensional PAGE showed that in the absence of PG (a) the PSII dimer was decomposed into monomers, and (b) the CP43 protein was detached from a major Part of th (en)
Title
  • Role of phosphatidylglycerol in the function and assembly of Photosystem II reaction center, studied in a cdsAinactivated PAL mutant strain of Synechocystis sp. PCC6803 that lacks phycobilisomes.
  • Role phosphatidylglycerolu ve funkci a skládání reakčního centra fotosystému II studovaná v mutantu PAL sinice Synechocystis PCC6803 s chybějícími fykobilisomy a s inaktivovaným genem cdsA (cs)
  • Role of phosphatidylglycerol in the function and assembly of Photosystem II reaction center, studied in a cdsAinactivated PAL mutant strain of Synechocystis sp. PCC6803 that lacks phycobilisomes. (en)
skos:prefLabel
  • Role of phosphatidylglycerol in the function and assembly of Photosystem II reaction center, studied in a cdsAinactivated PAL mutant strain of Synechocystis sp. PCC6803 that lacks phycobilisomes.
  • Role phosphatidylglycerolu ve funkci a skládání reakčního centra fotosystému II studovaná v mutantu PAL sinice Synechocystis PCC6803 s chybějícími fykobilisomy a s inaktivovaným genem cdsA (cs)
  • Role of phosphatidylglycerol in the function and assembly of Photosystem II reaction center, studied in a cdsAinactivated PAL mutant strain of Synechocystis sp. PCC6803 that lacks phycobilisomes. (en)
skos:notation
  • RIV/60076658:12640/08:00009350!RIV09-MSM-12640___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(IAA400200801), Z(AV0Z50200510), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika
  • x
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 393203
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12640/08:00009350
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Synechocystis sp PCC6803; phosphatidylglycerol; photosystem II; thermoluminescence; OJIP transient; cell division (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [6B5C8FBB227B]
http://linked.open...i/riv/nazevZdroje
  • Biochimica et Biophysica Acta - Bioenergetics
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 1777
http://linked.open...iv/tvurceVysledku
  • Komenda, Josef
  • Laczkó-Dobos, Hajnalka
  • Ughy, Bettina
  • Bogos, Balázs
  • Domonkos, Ildikó
  • Gombos, Zoltán
  • Itoh, Shigeru
  • Komura, Masayuki
  • Párducz, Árpád
  • Tóth, Szilvia Z
  • Zsiros, Ottó
http://linked.open...ain/vavai/riv/wos
  • 000259287200013
http://linked.open...n/vavai/riv/zamer
issn
  • 0005-2728
number of pages
http://localhost/t...ganizacniJednotka
  • 12640
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