About: Adipokinetic hormone-induced antioxidant response in Spodoptera littoralis

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The antioxidative potential of the Manduca sexta adipokinetic hormone (Manse-AKH) in the last instar larvae of Spodoptera littoralis (Noctuidae, Lepidoptera) was demonstrated after exposure to oxidative stress (OS) elicited by feeding on artificial diet containing tannic acid (TA). Determination of protein carbonyls (PCs) and reduced glutathione (GSH) levels, monitoring of activity of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferases (GSTs), as well as measuring of the mRNA expression of CAT and SOD were used as markers of the OS. Injection of the Manse-AKH (5 pmol per individual) reversed the OS status by mitigation of PCs formation and by stimulation of glutathione-S-transferases (GSTs) activity. The CAT and SOD mRNA expression was significantly suppressed after the Manse-AKH injection while activity of these enzymes was not affected. These results indicate that diminishing of OS after the AKH injection might be a result of activation of specific enzymatic pathway possibly at the post-translational level rather than a direct effect on regulation of antioxidant marker genes at the transcriptional level. The antioxidative potential of the Manduca sexta adipokinetic hormone (Manse-AKH) in the last instar larvae of Spodoptera littoralis (Noctuidae, Lepidoptera) was demonstrated after exposure to oxidative stress (OS) elicited by feeding on artificial diet containing tannic acid (TA). Determination of protein carbonyls (PCs) and reduced glutathione (GSH) levels, monitoring of activity of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferases (GSTs), as well as measuring of the mRNA expression of CAT and SOD were used as markers of the OS. Injection of the Manse-AKH (5 pmol per individual) reversed the OS status by mitigation of PCs formation and by stimulation of glutathione-S-transferases (GSTs) activity. The CAT and SOD mRNA expression was significantly suppressed after the Manse-AKH injection while activity of these enzymes was not affected. These results indicate that diminishing of OS after the AKH injection might be a result of activation of specific enzymatic pathway possibly at the post-translational level rather than a direct effect on regulation of antioxidant marker genes at the transcriptional level. (en)
Title	Adipokinetic hormone-induced antioxidant response in Spodoptera littoralis Adipokinetic hormone-induced antioxidant response in Spodoptera littoralis (en)
skos:prefLabel	Adipokinetic hormone-induced antioxidant response in Spodoptera littoralis Adipokinetic hormone-induced antioxidant response in Spodoptera littoralis (en)
skos:notation	RIV/60076658:12310/12:43883593!RIV13-MSM-12310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(GAP501/10/1215), V, Z(AV0Z50070508)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Kodrík, Dalibor Večeřa, Josef
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	121108
http://linked.open...ai/riv/idVysledku	RIV/60076658:12310/12:43883593
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Adipokinetic hormone; CABBAGE-LOOPER; LOCUSTA-MIGRATORIA; PERITROPHIC ENVELOPE; GLUTATHIONE-PEROXIDASE; SALMONELLA-TYPHIMURIUM; DROSOPHILA-MELANOGASTER; BACTERIAL LIPOPOLYSACCHARIDE; ESCHERICHIA-COLI; SUPEROXIDE-DISMUTASE; OXIDATIVE STRESS (en)
http://linked.open.../riv/klicoveSlovo	Adipokinetic hormone BACTERIAL LIPOPOLYSACCHARIDE CABBAGE-LOOPER DROSOPHILA-MELANOGASTER GLUTATHIONE-PEROXIDASE LOCUSTA-MIGRATORIA PERITROPHIC ENVELOPE SALMONELLA-TYPHIMURIUM SUPEROXIDE-DISMUTASE ESCHERICHIA-COLI OXIDATIVE STRESS
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[68057CA342EB]
http://linked.open...i/riv/nazevZdroje	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Stress hormones and their potential usage in insect pest control
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	155
http://linked.open...iv/tvurceVysledku	Kodrík, Dalibor Večeřa, Josef Krishnan, Natraj Mithoefer, Axel Vogel, Heiko
http://linked.open...ain/vavai/riv/wos	000299580200028
http://linked.open...n/vavai/riv/zamer	Study of the regulation of insect organism, dynamics of insect populations and function of insects in ecosystems
issn	1532-0456
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.cbpc.2011.10.009
http://localhost/t...ganizacniJednotka	12310
is http://linked.open...avai/riv/vysledek of	Adipokinetic hormone-induced antioxidant response in Spodoptera littoralis

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