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  • We show design of a microscope slide-based microarray assay for simultaneous identification of six potato viruses. Primer pairs were designed to obtain specific amplicons from: Potato virus A, S, X, Y, Potato mop-top virus and Potato leaf-roll virus. Viral DNA probes were spotted on a microscope slide coated with poly-L-lysine. The same primers were used for preparation of fluorochrome-labeled targets. The latter were hybridized on the microarray slide. Simultaneous assay of two pathogens is given.
  • We show design of a microscope slide-based microarray assay for simultaneous identification of six potato viruses. Primer pairs were designed to obtain specific amplicons from: Potato virus A, S, X, Y, Potato mop-top virus and Potato leaf-roll virus. Viral DNA probes were spotted on a microscope slide coated with poly-L-lysine. The same primers were used for preparation of fluorochrome-labeled targets. The latter were hybridized on the microarray slide. Simultaneous assay of two pathogens is given. (en)
  • DNA microarray: paralelní detekce virů brambor (cs)
Title
  • DNA microarray: paralelní detekce virů brambor (cs)
  • DNA microarray: parallel detection of potato viruses
  • DNA microarray: parallel detection of potato viruses (en)
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  • DNA microarray: paralelní detekce virů brambor (cs)
  • DNA microarray: parallel detection of potato viruses
  • DNA microarray: parallel detection of potato viruses (en)
skos:notation
  • RIV/60076658:12310/03:00008570!RIV08-MSM-12310___
http://linked.open.../vavai/riv/strany
  • 41-44
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/01/1105), V, Z(AV0Z5051902)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
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  • 604247
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12310/03:00008570
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • DNA microarray assay; potato viruses; diagnostics (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • SK - Slovenská republika
http://linked.open...ontrolniKodProRIV
  • [D8DD96AD80A0]
http://linked.open...i/riv/nazevZdroje
  • Acta Virologica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...v/svazekPeriodika
  • 47
http://linked.open...iv/tvurceVysledku
  • Lenz, Ondřej
  • Mráz, I.
  • Dědič, P.
  • Bystřická, Dagmar
  • Šíp, Miroslav
http://linked.open...n/vavai/riv/zamer
issn
  • 0001-723X
number of pages
http://localhost/t...ganizacniJednotka
  • 12310
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