About: Research on molecular organization of plant genome, analysis of targeted changes and expression of heritability in interaction with environment and pathogens

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Zamer, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Zamer
Description	Cílem záměru je poznání strukturního a prostorového uspořádání genomu rostlin, vývoj nových postupů izolace, modifikace a přenosu genů, diagnostiky fytopatogenů, poznání struktury, funkce a změn pigment-bílkovinných komplexů a metabolismu bakteriálních fytopatogenů. Cíle budou dosaženy přípravou chromozómově specifických sond a fyzikálním mapování genů, studiem chování transponovatelných elementů, izolací a modifikací morforegulačních rostlinných genů a morfologickou a anatomickou charakterizací mutantů počítačovou analýzou. Využita bude variabilita virových, fytoplazmových a viroidních genomů a působení protismyslových nukleových kyselin cílených proti virům a viroidům. Výsledkem bude poznání evolučních tendencí rostlinného genomu, identifikace a lokalizace genů a nové metody jejich přenosu, využitelné k získání odrůd s agronomicky významnými vlastnostmi včetně odolností k patogenům. Molekulární markery budou využity ke zpřesnění diagnostiky patogenů a identifikaci odrůd. The research will be aimed on compositional and spatial organization of plant genome, development of new approaches for isolation, modification and transfer of genes, diagnostics of phytopathogens, understanding of the structure, function and changes of pigment-protein complexes of plants, and study of metabolism of bacterial phytopathogens. The strategies will be based on preparation of chromosome-specific probes, physical gene mapping, characterization of the behaviour of transposable elements, isolation and modification of morphoregulatory genes, characterization of mutants by computer image analysis, and exploitation of variability of plant viruses, phytoplasmas and viroids and antisense nucleic acids targeted against viroids and viruses. Results enable to recognise evolutionary trends of plant genome, isolate and transfer of selected genes, create new cultivars with agronomically improved traits and resistances, and specify molecular diagnostics of phytopathogens and cultivars. (en)
Title	Research on molecular organization of plant genome, analysis of targeted changes and expression of heritability in interaction with environment and pathogens (en) Výzkum molekulárního uspořádání dědičné informace rostlin a analýza cílených změn a projevů dědičnosti v interakci s prostředím a patogeny
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/zamer/AV0Z5051902/celkovaStatniPodpora
http://linked.open...ez/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/zamer/AV0Z5051902/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2005-06-17 (xsd:date)
http://linked.open...vavai/cez/dodavka	http://linked.opendata.cz/resource/domain/vavai/dodavka/CEZ%2F2005%2FAV0%2FAV0500%2FU%2FN%2F3%3A1
http://linked.open...ez/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...in/vavai/cez/faze	33123777
http://linked.open...ai/cez/hlavniObor	EB - Genetika a molekulární biologie
http://linked.open...vai/cez/hodnoceni	U - Uspěl podle zadání, tj. byly splněny cíle a jeho předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory
http://linked.open.../cez/kategorieVaV	ZV - Základní výzkum
http://linked.open.../cez/klicovaSlova	plant;genome;organization;nucleic;acid;gene;isolation;modification;transfer;physical;mapping;molecular;markers;morphology;mutants;probes;virus;viroid;fytoplasma;diagnosis;photosynthesis;bacterial phytopathogens;transposable element (en)
http://linked.open...icUkonceniPodpory	1 (xsd:int)
http://linked.open...icZahajeniPodpory	1 (xsd:int)
http://linked.open...cez/partnerHlavni	Ústav molekulární biologie rostlin AV ČR
http://linked.open...z/poceVysledkuRIV	125 (xsd:int)
http://linked.open...inujicichPrijemcu	0 (xsd:int)
http://linked.open...cez/pocetPrijemcu	1 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	0 (xsd:int)
http://linked.open...enychVysledkuVRIV	125 (xsd:int)
http://linked.open.../vavai/cez/resort	Academy of Sciences of the Czech Republic
http://linked.open...okUkonceniPodpory	2004
http://linked.open...okZahajeniPodpory	1999
http://linked.open...iciPoslednihoRoku	2005
http://linked.open...atUdajeProjZameru	2005
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open.../cez/vedlejsiObor	EI - Biotechnologie a bionika GE - Šlechtění rostlin
http://linked.open...n/vavai/cez/zamer	Z - Výzkumný záměr financovaný ze státního rozpočtu
http://linked.open...sledkuDodavatelem	Novel plant mobile elements were found, antisense nucleic acids against viroids and viruses, modification of morphoregulatory genes, variability of plant viruses and phytoplasmas and regulation of photosynthetic processes in photosystem II were studied. (en) Byly izolovány nové mobilní elementy rostlin, využity protismyslové nukleové kyseliny proti viroidům a virům, modifikovány morforegulační geny, zkoumána variabilita rostlinných virů a fytoplazem a analyzovány fotosyntetické procesy ve fotosystému II. (cs)
http://linked.open...cez/zivotniCyklus	ZBBBBKU
is http://linked.open...n/vavai/riv/zamer of	The establishment and screening of hop genomic and cDNA libraries for genes determining lupulin production Název česky Založení a skrínink chmelové genomové a cDNA knihovny s geny determinujícími produkci lupulinu Study of infection and sequence variability of viroids in CR Limitations in reliability of fire blight (Erwinia amylovora) diagnostic methods Carotenoid excited states - photophysics, ultrafast dynamics and photosynthetic functions Structure, divergence, and distribution of the CRR centromeric retrotransposon family in rice Zaba: a novel miniature transposable element present in genomes of legume plants. Development of a composite map in Vicia faba, breeding applications and future prospects Tuning energy transfer in the peridinin-chlorophyll complex by reconstitution with different chlorophylls First attempt on identification of phytoplasma associated with full blossom of red and white currant in the Czech Republic. Comparison of hCMV immediate early and CaMV 35S promoters in both plant and human cells Transposition patterns of unlinked transposed Ds elements from two T-DNA loci on tomato chromosomes 7 and 8. Transgenose rostlin. Energy transfer and conformational dynamics in Zn-porphyrin dendrimers Chromosome analysis and sorting in Vicia sativa using flow cytometry Methods and prospects of flax transgenosis. Photoadaptation in the green alga Spongiochloris sp. A three-fluorometer study. Introduction to plant transgenosis. Germinal excision and reinsertion frequencies of the mobile elements Ds transposed from two unlinked T-DNA loci in tomato. First report on a yellows disease of allium altyncolium associated with phytoplasma infection. Identification of phytoplasma infecting winter oilseed rape in the Czech Republic and its comparison with Italian Brassica phytoplasmas. New antisense RNA systems targeted against plant pathogens. Molecular variability of the capsid protein of the prune dwarf virus. Quantitative and sequence-specific analysis of DNA-ligand interaction by means of fluorescent intercalator probes. Quenching of chlorophyll a fluorescence in the micellar system containing selected aromatic compounds. Occurence of graft-transmissible of the strawberry in the Czech Republic. The occurence of strawberry viruses and phytoplasmas in the Czech Republic. Outbreak of full blossom of red currant in the Czech Republic. The variability of hop latent viroid as induced upon heat-treatment. Transgenosis of Arabidopsis thaliana for undestanding gene functions and interactions. Timing of transposition of Ac mobile element in potato. Variability and molecular typing of the woody-tree infecting ilaviruses (Prunus necrotic ringspot virus and Prune dwarf virus. Analysis of true chalcone synthase from Humulus lupulus L. and biotechnology aspects of 'medicinal hops'. Analysis of transcription of plant 7SL RNA gene variants in HeLa in vitro transcription system. Development and characterization of microsatellite markers from chromosome 1-specific DNA libraries of Vicia faba. Field resistance of six cultivars of winter oilseed rape against Turnip yellow mosiac virus. Further investigation on full blossom disease of currants in the Czech Republic. Generative organs of higher plants. Resistance of transgenic tobacco seedlings expressing the Agrobacterium tumefaciens C58-6b gene, to growth-inhibitory levels of cytokinin is associated with elevated IAA levels and activation of phenylpropanoid metabolism. The promiscuity of heterospecific lox sites increases dramatically in the presence of palindromic DNA. Reconstruction of DNA shape from AFM data. Remarkable variability of apple mosiac virus capsid protein gene after nucleotide position 141. DNA microarray: parallel detection of potato viruses Electron microscopy in structural studies of Photosystem II. Electron microscopy and molecular characterization of phytoplasmas associated with strawflower yellows in the Czech Republic. Effect of resolving medium and staining procedure on inter-simple-sequence-repeat (ISSR) marker patterns in flax. Studies of phospholipid binding to N-terminal domain of membrane protein light-harvesting complex II Two new families of tandem repeats isolated from genus Vicia using genomic self-priming PCR. Variability and molecular typing of the woody-tree infecting prunus necrotic ringspot ilavirus. A molecular survey to identify phytopalasmas associated with apple trees showing different diseases symptoms. Activities of ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase and oxygen evolution in transgenic tobacco plants. Association of aster yelow subgroup 16SrI-B phytoplasmas with a disease of Rehmannia glutinosa var. purpurea. Printing DNA microarrays using Biomek Laboratory Automation Workstation. Preparation of recombinant coat protein of Prunus necrotic ringspot virus. Cloning and characterisation of chs-specific DNA and cDNA sequences from hop (Humulus lupulus L.). Comparison of genetic variability between Czech and foreign isolates of phytopathogenic bacteria Clavibacter michiganensis subsp. sepedonicus by Rep-PCR technique. Chromosome sorting and PCR-based physical mapping in pea (Pisum sativum L.). Chlorophyll fluorescence parameters: the definitions, photosynthetic meaning, and mutual relationships. Excitonic interactions in the reaction centre of photosystem II studied by using circular dichroism. Immunocapture RT-PCR probing of potato virus Y isolates. Mixed infection of black currant (Ribes Nigrum L.) plants with blackcurrant reversion associated virus and rhabdovirus-like particles with symptoms of black currant reversion disease. PlantSat: a specialized database for plant satellite repeats. Polyacrylamide gel polymerization with adjustable gelation rate. Plant 7SL RNA and tRNA(Tyr) genes with inserted antisense sequences are efficiently expressed in an in vitro transcription system from Nicotiana tabacum cells. Photosystem II reaction center with altered pigment-composition: reconstitution of a complex containing five chlorophyll a per two pheophytin a with modified chlorophylls. A nonspecific, single-stranded nuclease activity with characteristics of a topoisomerase found in a major grass pollen allergen: Possible biological significance.

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