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  • Fire blight, caused by Erwinia amylovora, is an infectious disease affecting pome fruit and other members of the rose family. It is a serious concern to producers of apples and pears in the Czech Republic. Under optimal conditions, it can seriously damage an entire apple or pear orchard in a growing season. Resistance of old local pome fruit cultivars to E. amylovora was tested in an in vitro culture assay. Four pear cultivars (´Belle de Bois´, ´Jakubka ceska´, ´Doynne de Merode´, ´Solanka´) and two apple cultivars (´Boiken´, ´Nonnetit´) were selected for testing. A verified isolate of E. amylovora (Ea 8/95) isolated from necrotic tissues of hawthorn (Crataegus x monogyna) was used in tests. The apical tip of the shoot was removed with scissors and two concentrations of the pathogen (103 and 106 cfu/ml) were applied on the cut surface. Based on the results, ´Solanka´ was the most resistant and ´Doynne de Merode´ the most susceptible of the selected pear cultivars. Concerning apple, two cultivars ´Boiken´ and ´Nonnetit´ were tested. In this test, cultivar ´Boiken´ was three times more susceptible than ´Nonnetit´. ´Nonnetit´ showed minor symptoms of necrosis 9 days after inoculation. Preliminary results from inoculation of pear and apple shoot tips maintained in tissue culture with E. amylovora was a useful method to test fire blight resistance in pome fruit cultivars. The obtained results are preliminary and evaluation of additional cultivars will be carried out in following years
  • Fire blight, caused by Erwinia amylovora, is an infectious disease affecting pome fruit and other members of the rose family. It is a serious concern to producers of apples and pears in the Czech Republic. Under optimal conditions, it can seriously damage an entire apple or pear orchard in a growing season. Resistance of old local pome fruit cultivars to E. amylovora was tested in an in vitro culture assay. Four pear cultivars (´Belle de Bois´, ´Jakubka ceska´, ´Doynne de Merode´, ´Solanka´) and two apple cultivars (´Boiken´, ´Nonnetit´) were selected for testing. A verified isolate of E. amylovora (Ea 8/95) isolated from necrotic tissues of hawthorn (Crataegus x monogyna) was used in tests. The apical tip of the shoot was removed with scissors and two concentrations of the pathogen (103 and 106 cfu/ml) were applied on the cut surface. Based on the results, ´Solanka´ was the most resistant and ´Doynne de Merode´ the most susceptible of the selected pear cultivars. Concerning apple, two cultivars ´Boiken´ and ´Nonnetit´ were tested. In this test, cultivar ´Boiken´ was three times more susceptible than ´Nonnetit´. ´Nonnetit´ showed minor symptoms of necrosis 9 days after inoculation. Preliminary results from inoculation of pear and apple shoot tips maintained in tissue culture with E. amylovora was a useful method to test fire blight resistance in pome fruit cultivars. The obtained results are preliminary and evaluation of additional cultivars will be carried out in following years (en)
Title
  • Testing of Resistance to Erwinia amylovora in an In Vitro Culture Assay
  • Testing of Resistance to Erwinia amylovora in an In Vitro Culture Assay (en)
skos:prefLabel
  • Testing of Resistance to Erwinia amylovora in an In Vitro Culture Assay
  • Testing of Resistance to Erwinia amylovora in an In Vitro Culture Assay (en)
skos:notation
  • RIV/25271121:_____/11:#0000191!RIV13-MZE-25271121
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QH91148)
http://linked.open...iv/cisloPeriodika
  • 896
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 234922
http://linked.open...ai/riv/idVysledku
  • RIV/25271121:_____/11:#0000191
http://linked.open...riv/jazykVysledku
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  • fire blight; bacterium; apple; pear; infection (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • BE - Belgické království
http://linked.open...ontrolniKodProRIV
  • [2C44A0ACFA25]
http://linked.open...i/riv/nazevZdroje
  • Acta Horticulturae
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Korba, Josef
  • Paprštein, František
  • Sedlák, Jiří
  • Šillerová, Jana
issn
  • 0567-7572
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