About: In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm
Description	The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), to perform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential male components in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secretome. The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), to perform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential male components in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secretome. (en)
Title	In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception (en)
skos:prefLabel	In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception (en)
skos:notation	RIV/00216224:14740/14:00073698!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0068), P(GAP501/11/1462), P(GPP501/11/P321), P(LD13049), S
http://linked.open...iv/cisloPeriodika	April
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Zdráhal, Zbyněk Potěšil, David
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	21048
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00073698
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	GPI-ANCHORED PROTEIN; PLANT SECRETOME; ARABIDOPSIS; GROWTH; GUIDANCE; GERMINATION; ATTRACTANT; COMPONENTS; MAIZE; CELL (en)
http://linked.open.../riv/klicoveSlovo	COMPONENTS GROWTH CELL ARABIDOPSIS MAIZE ATTRACTANT GERMINATION GPI-ANCHORED PROTEIN GUIDANCE PLANT SECRETOME
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[AE6B3A0FB0BA]
http://linked.open...i/riv/nazevZdroje	BIOCHEMICAL SOCIETY TRANSACTIONS
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Localisation of translation of cell wall components in growing pollen tube, an effectively single-cell model system Depicting a functional model of NTP303 mRNA translational repression during tobacco pollen maturation Functional characterisation of pollen unique storage ribonucleoprotein particles
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	42
http://linked.open...iv/tvurceVysledku	Zdráhal, Zbyněk Fíla, Jan Honys, David Čapková, Věra Hafidh, Said Potěšil, David Fecíková, Jana
http://linked.open...ain/vavai/riv/wos	000333658900028
issn	0300-5127
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1042/BST20130204
http://localhost/t...ganizacniJednotka	14740

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