About: Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186968/
Description	The Nrd1-Nab3-Sen1 (NNS) complex is essential for controlling pervasive transcription and generating sn/snoRNAs in S. cerevisiae. The NNS complex terminates transcription of noncoding RNA genes and promotes exosome-dependent processing/degradation of the released transcripts. The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation. NNS-dependent termination and degradation are coupled, but the mechanism underlying this coupling remains enigmatic. Here we provide structural and functional evidence demonstrating that the same domain of Nrd1p interacts with RNA polymerase II and Trf4p in a mutually exclusive manner, thus defining two alternative forms of the NNS complex, one involved in termination and the other in degradation. We show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro. The Nrd1-Nab3-Sen1 (NNS) complex is essential for controlling pervasive transcription and generating sn/snoRNAs in S. cerevisiae. The NNS complex terminates transcription of noncoding RNA genes and promotes exosome-dependent processing/degradation of the released transcripts. The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation. NNS-dependent termination and degradation are coupled, but the mechanism underlying this coupling remains enigmatic. Here we provide structural and functional evidence demonstrating that the same domain of Nrd1p interacts with RNA polymerase II and Trf4p in a mutually exclusive manner, thus defining two alternative forms of the NNS complex, one involved in termination and the other in degradation. We show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro. (en)
Title	Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation (en)
skos:prefLabel	Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation (en)
skos:notation	RIV/00216224:14740/14:00073613!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(EE2.3.20.0042), P(GA13-18344S), P(GBP305/12/G034)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Fořtová, Andrea Kubíček, Karel Vaňáčová, Štěpánka Štefl, Richard Kabzinski, Tomasz
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	30269
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00073613
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CRYPTIC UNSTABLE TRANSCRIPTS; POLYMERASE-II TRANSCRIPTS; BINDING PROTEINS NRD1; QUALITY-CONTROL; SACCHAROMYCES-CEREVISIAE; PERVASIVE TRANSCRIPTION; BIDIRECTIONAL PROMOTERS; POLY(A) POLYMERASE; NUCLEAR EXOSOME; TRAMP COMPLEX (en)
http://linked.open.../riv/klicoveSlovo	BINDING PROTEINS NRD1 CRYPTIC UNSTABLE TRANSCRIPTS NUCLEAR EXOSOME POLY(A) POLYMERASE POLYMERASE-II TRANSCRIPTS TRAMP COMPLEX BIDIRECTIONAL PROMOTERS PERVASIVE TRANSCRIPTION SACCHAROMYCES-CEREVISIAE QUALITY-CONTROL
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[56AFFEF6C33C]
http://linked.open...i/riv/nazevZdroje	Molecular Cell
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for RNA Biology Central european institute of technology Internationalisation of the Structural Biology Research Programme with Emphasis on New Directions in R&D Studium kódu CTD RNA polymerázy II
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Fořtová, Andrea Kubíček, Karel Vaňáčová, Štěpánka Štefl, Richard Libri, Domenico Cramer, Patrick Fuerte, Odil Porrua Kabzinski, Tomasz Lacroute, Francoise Lidschreiber, Michael Tudek, Agnieszka
http://linked.open...ain/vavai/riv/wos	000340646600012
issn	1097-2765
number of pages	15 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.molcel.2014.05.031
http://localhost/t...ganizacniJednotka	14740

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