About: Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://nar.oxfordjournals.org/content/early/2012/11/26/nar.gks1135.full
Description	DNA concentration has been recently suggested to be the reason why different arrangements are revealed for K+-stabilized human telomere quadruplexes by experimental methods requiring DNA concentrations differing by orders of magnitude. As Raman spectroscopy can be applied to DNA samples ranging from those accessible by absorption and CD spectroscopies up to extremely concentrated solutions, gels and even crystals; it has been used here to clarify polymorphism of a core human telomeric sequence G3(TTAG3)3 in the presence of K+and Na+ ions throughout wide range of DNA concentrations. We demonstrate that the K+-structure of G3(TTAG3)3 at low DNA concentration is close to the antiparallel fold of Na+-stabilized quadruplex. On the increase of G3(TTAG3)3 concentration, a gradual transition from antiparallel to intramolecular parallel arrangement was observed, but only for thermodynamically equilibrated K+-stabilized samples. The transition is synergically supported by increased K+ concentration. DNA concentration has been recently suggested to be the reason why different arrangements are revealed for K+-stabilized human telomere quadruplexes by experimental methods requiring DNA concentrations differing by orders of magnitude. As Raman spectroscopy can be applied to DNA samples ranging from those accessible by absorption and CD spectroscopies up to extremely concentrated solutions, gels and even crystals; it has been used here to clarify polymorphism of a core human telomeric sequence G3(TTAG3)3 in the presence of K+and Na+ ions throughout wide range of DNA concentrations. We demonstrate that the K+-structure of G3(TTAG3)3 at low DNA concentration is close to the antiparallel fold of Na+-stabilized quadruplex. On the increase of G3(TTAG3)3 concentration, a gradual transition from antiparallel to intramolecular parallel arrangement was observed, but only for thermodynamically equilibrated K+-stabilized samples. The transition is synergically supported by increased K+ concentration. (en)
Title	Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study (en)
skos:prefLabel	Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study (en)
skos:notation	RIV/00216224:14740/13:00067549!RIV14-MSM-14740___
http://linked.open...avai/predkladatel	Středoevropský technologický institut
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0068), P(GA202/09/0193), P(GAP205/12/0466), S, Z(AV0Z50040702), Z(MSM0021620835)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Vorlíčková, Michaela Kejnovská, Iva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	97098
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/13:00067549
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	NTRAMOLECULAR G-QUADRUPLEX; K+ SOLUTION; B-DNA; MELTING TRANSITIONS; POTASSIUM SOLUTION; POLARIZED RAMAN; NUCLEIC-ACIDS; SPECTROSCOPY; SEQUENCE; TETRAPLEX (en)
http://linked.open.../riv/klicoveSlovo	SEQUENCE SPECTROSCOPY K+ SOLUTION B-DNA NUCLEIC-ACIDS MELTING TRANSITIONS NTRAMOLECULAR G-QUADRUPLEX POLARIZED RAMAN POTASSIUM SOLUTION TETRAPLEX
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[C769E9BD8344]
http://linked.open...i/riv/nazevZdroje	Nucleic Acids Research
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Formation and dynamics of nucleic acid motifs involved in regulation of gene expression DNA quadruplexes in the human genome associated with diseases and aging
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	41
http://linked.open...iv/tvurceVysledku	Mojzeš, Peter Vorlíčková, Michaela Palacký, Jan Kejnovská, Iva
http://linked.open...ain/vavai/riv/wos	000314121100034
http://linked.open...n/vavai/riv/zamer	Genome and epigenome: 1D and 3D Structure, Dynamics, Interactions with Proteins and Functions Fyzika molekulárních, makromolekulárních a biologických systémů
issn	0305-1048
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1093/nar/gks1135
http://localhost/t...ganizacniJednotka	14740

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