About: The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling     Goto   Sponge   NotDistinct   Permalink

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Description
  • For the purpose of this study, we used an extraction protocol recommended for clinical laboratories for MALDI-TOF MS profiling of bacteria. Seventeen closely related strains of the Lactobacillus acidophilus group were cultivated under various growth conditions (growth medium, time, and temperature) and analyzed. Out of a total of 327 samples, 80 % were correctly assigned to the species level and 13 % only to the genus level. When using data obtained from strains cultured for lengthy periods (7 days), the identification success rate was reduced due to poor signal quality, whereas with shorter cultivation times there was no influence of growth conditions on the assignment of particular strains to their corresponding species. However, variations in certain cultivation parameters were found to influence identification and differentiation of most of the examined strains. Strain discrimination was frequently found to be dependent on the selection of culture conditions.
  • For the purpose of this study, we used an extraction protocol recommended for clinical laboratories for MALDI-TOF MS profiling of bacteria. Seventeen closely related strains of the Lactobacillus acidophilus group were cultivated under various growth conditions (growth medium, time, and temperature) and analyzed. Out of a total of 327 samples, 80 % were correctly assigned to the species level and 13 % only to the genus level. When using data obtained from strains cultured for lengthy periods (7 days), the identification success rate was reduced due to poor signal quality, whereas with shorter cultivation times there was no influence of growth conditions on the assignment of particular strains to their corresponding species. However, variations in certain cultivation parameters were found to influence identification and differentiation of most of the examined strains. Strain discrimination was frequently found to be dependent on the selection of culture conditions. (en)
Title
  • The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling
  • The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling (en)
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  • The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling
  • The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling (en)
skos:notation
  • RIV/00216224:14740/13:00066925!RIV14-MSM-14740___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
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  • P(ED1.1.00/02.0068), P(GA13-26693S), S
http://linked.open...iv/cisloPeriodika
  • 24
http://linked.open...vai/riv/dodaniDat
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  • 79783
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  • RIV/00216224:14740/13:00066925
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  • DESORPTION-IONIZATION-TIME; MALDI-TOF-MS; LACTIC-ACID BACTERIA; STAPHYLOCOCCUS-AUREUS; RAPID IDENTIFICATION; MICROORGANISM IDENTIFICATION; WHOLE CELLS; CLASSIFICATION; DISCRIMINATION; CULTURE (en)
http://linked.open.../riv/klicoveSlovo
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  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [539733E95442]
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  • Rapid Communications in Mass Spectrometry
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http://linked.open...ichTvurcuVysledku
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http://linked.open...v/svazekPeriodika
  • 27
http://linked.open...iv/tvurceVysledku
  • Tvrzová, Ludmila
  • Zdráhal, Zbyněk
  • Šedo, Ondrej
  • Medková, Marta
  • Vávrová, Andrea
http://linked.open...ain/vavai/riv/wos
  • 000330107600001
issn
  • 0951-4198
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/rcm.6741
http://localhost/t...ganizacniJednotka
  • 14740
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