About: Analysis of the Nse3/MAGE-binding Domain of the Nse4/EID Family Proteins

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About: Analysis of the Nse3/MAGE-binding Domain of the Nse4/EID Family Proteins Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035813
Description	Background: The Nse1, Nse3 and Nse4 proteins form a tight sub-complex of the large SMC5-6 protein complex. hNSE3/MAGEG1, the mammalian ortholog of Nse3, is the founding member of the MAGE (melanoma-associated antigen) protein family and the Nse4 kleisin subunit is related to the EID (E1A-like inhibitor of differentiation) family of proteins. We have recently shown that human MAGE proteins can interact with NSE4/EID proteins through their characteristic conserved hydrophobic pocket. Methodology/Principal Findings: Using mutagenesis and protein-protein interaction analyses, we have identified a new Nse3/MAGE-binding domain (NMBD) of the Nse4/EID proteins. This short domain is located next to the Nse4 N-terminal kleisin motif and is conserved in all NSE4/EID proteins. The central amino acid residues of the human NSE4b/EID3 domain were essential for its binding to hNSE3/MAGEG1 in yeast two-hybrid assays suggesting they form the core of the binding domain. Background: The Nse1, Nse3 and Nse4 proteins form a tight sub-complex of the large SMC5-6 protein complex. hNSE3/MAGEG1, the mammalian ortholog of Nse3, is the founding member of the MAGE (melanoma-associated antigen) protein family and the Nse4 kleisin subunit is related to the EID (E1A-like inhibitor of differentiation) family of proteins. We have recently shown that human MAGE proteins can interact with NSE4/EID proteins through their characteristic conserved hydrophobic pocket. Methodology/Principal Findings: Using mutagenesis and protein-protein interaction analyses, we have identified a new Nse3/MAGE-binding domain (NMBD) of the Nse4/EID proteins. This short domain is located next to the Nse4 N-terminal kleisin motif and is conserved in all NSE4/EID proteins. The central amino acid residues of the human NSE4b/EID3 domain were essential for its binding to hNSE3/MAGEG1 in yeast two-hybrid assays suggesting they form the core of the binding domain. (en)
Title	Analysis of the Nse3/MAGE-binding Domain of the Nse4/EID Family Proteins Analysis of the Nse3/MAGE-binding Domain of the Nse4/EID Family Proteins (en)
skos:prefLabel	Analysis of the Nse3/MAGE-binding Domain of the Nse4/EID Family Proteins Analysis of the Nse3/MAGE-binding Domain of the Nse4/EID Family Proteins (en)
skos:notation	RIV/00216224:14740/12:00057090!RIV13-GA0-14740___
http://linked.open...avai/predkladatel	Středoevropský technologický institut
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD204/08/H054), P(IAA501630902), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Paleček, Jan Bednářová, Kateřina Kozáková, Lucie Janoš, Pavel Kříž, Zdeněk Guérineau, Marc
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	122281
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/12:00057090
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	SMC5-6 complex subunits; Nse4/EID; Nse3/MAGE/necdin; protein-protein docking; protein co-evolution (en)
http://linked.open.../riv/klicoveSlovo	Nse4/EID Nse3/MAGE/necdin SMC5-6 complex subunits protein co-evolution protein-protein docking
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F29615F99049]
http://linked.open...i/riv/nazevZdroje	PLoS One
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Characterization of MAGE proteins: cofactors for E3 ubiquitin ligases? Molecular mechanisms of the cell proliferation and differentiation
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Kříž, Zdeněk Bednářová, Kateřina Guérineau, Marc Kozáková, Lucie Paleček, Jan Janoš, Pavel
http://linked.open...ain/vavai/riv/wos	000305339200115
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
issn	1932-6203
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0035813
http://localhost/t...ganizacniJednotka	14740
is http://linked.open...avai/riv/vysledek of	Analysis of the Nse3/MAGE-binding Domain of the Nse4/EID Family Proteins

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