About: Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The influence of the matrix solution, sample form and deposition technique on the quality MALDI-TOF mass spectra was examined and assessed with the aim to improve MALDI-TOF MS performance for the identification of microorganisms and to enable automatic spectra acquisition. It was observed that the use of matrix compounds ferulic and sinapinic acid may result in improved mass spectral features, in terms of signal resolution and S/N ratio, as compared to alpha-cyano-4-hydroxycinnamic acid, which was, on the other hand, found to be the only matrix compound that enabled fully automatic mass spectra acquisition. The robustness of the whole sample preparation procedure was then assessed on a set of 25 strains of four Acinetobacter species. Results showed reproducible detection of subtle mass spectral differences between strains belonging to the same species, although they do not confirm the possibility of reliable strain typing. The influence of the matrix solution, sample form and deposition technique on the quality MALDI-TOF mass spectra was examined and assessed with the aim to improve MALDI-TOF MS performance for the identification of microorganisms and to enable automatic spectra acquisition. It was observed that the use of matrix compounds ferulic and sinapinic acid may result in improved mass spectral features, in terms of signal resolution and S/N ratio, as compared to alpha-cyano-4-hydroxycinnamic acid, which was, on the other hand, found to be the only matrix compound that enabled fully automatic mass spectra acquisition. The robustness of the whole sample preparation procedure was then assessed on a set of 25 strains of four Acinetobacter species. Results showed reproducible detection of subtle mass spectral differences between strains belonging to the same species, although they do not confirm the possibility of reliable strain typing. (en)
Title	Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species (en)
skos:prefLabel	Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species (en)
skos:notation	RIV/00216224:14740/11:00052199!RIV12-MZE-14740___
http://linked.open...avai/predkladatel	Středoevropský technologický institut
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B08060), P(LC06034), P(QI101A094), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Zdráhal, Zbyněk Šedo, Ondrej Voráč, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	218758
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/11:00052199
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Acinetobacter sp.; bacteria identification; MALDI-TOF MS (en)
http://linked.open.../riv/klicoveSlovo	Acinetobacter sp. bacteria identification MALDI-TOF MS
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[2B18ECF14DA1]
http://linked.open...i/riv/nazevZdroje	Systematic and Applied Microbiology
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Methods of eradication of production diseases of cattle - bovine viral diarrhoea - mucosal disease and paratuberculosis Regulation of morphogenesis of plant cells and organs Development of diagnostics and treatment of serious heart and vascular diseases using genomic and proteomic approaches
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	34
http://linked.open...iv/tvurceVysledku	Zdráhal, Zbyněk Šedo, Ondrej Voráč, Aleš
http://linked.open...ain/vavai/riv/wos	000289395200005
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
issn	0723-2020
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.syapm.2010.11.008
http://localhost/t...ganizacniJednotka	14740
is http://linked.open...avai/riv/vysledek of	Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software