About: Directed evolution of lectins from pathogenic bacteria

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Directed evolution is widely used in protein engineering for producing proteins with altered or improved properties. This powerful tool includes several main approaches. The first is a random mutagenesis, which is used for creating random mutations anywhere in the gene of interest. The second is a targeted random mutagenesis, which is utilized for preparing mutants with random base substitutions in selected hotspots. In addition, a site directed mutagenesis is based on rational design and it serves for creating specific amino acid substitutions in selected hotspots. In our work two technical approaches - the targeted-random mutagenesis via mixture of oligonucleotide primers and the site directed mutagenesis via two primers carrying specific substitution – are employed. Lectins are proteins produced by different organisms. They are able to bind various sugars specifically. Directed evolution is widely used in protein engineering for producing proteins with altered or improved properties. This powerful tool includes several main approaches. The first is a random mutagenesis, which is used for creating random mutations anywhere in the gene of interest. The second is a targeted random mutagenesis, which is utilized for preparing mutants with random base substitutions in selected hotspots. In addition, a site directed mutagenesis is based on rational design and it serves for creating specific amino acid substitutions in selected hotspots. In our work two technical approaches - the targeted-random mutagenesis via mixture of oligonucleotide primers and the site directed mutagenesis via two primers carrying specific substitution – are employed. Lectins are proteins produced by different organisms. They are able to bind various sugars specifically. (en)
Title	Directed evolution of lectins from pathogenic bacteria Directed evolution of lectins from pathogenic bacteria (en)
skos:prefLabel	Directed evolution of lectins from pathogenic bacteria Directed evolution of lectins from pathogenic bacteria (en)
skos:notation	RIV/00216224:14740/11:00050138!RIV12-GA0-14740___
http://linked.open...avai/predkladatel	Středoevropský technologický institut
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA303/09/1168), P(GD301/09/H004), P(LC06030), S, Z(MSM0021622413)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Pokorná, Martina Safy, Marek Wimmerová, Michaela Mrázková, Jana
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	194761
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/11:00050138
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	directed evolution; random mutagenesis in vitro; targeted-random mutagenesis; site-directed mutagenersis; lectins; PA-IIL; Pseudomonas aeruginosa; RS-IIL; Ralstonia solanacearum; BC2L-A; Burkholderia cenocepacia (en)
http://linked.open.../riv/klicoveSlovo	lectins Pseudomonas aeruginosa BC2L-A PA-IIL RS-IIL Ralstonia solanacearum directed evolution random mutagenesis in vitro site-directed mutagenersis targeted-random mutagenesis Burkholderia cenocepacia
http://linked.open...ontrolniKodProRIV	[5EF2CA98992F]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Lectins from human pathogens - structure, function, engineering Molecular and structural biology of selected antitumor drugs. From mechanistic studies to chemotherapy of tumors Biomolecular centre
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Mrázková, Jana Pokorná, Martina Wimmerová, Michaela Safy, Marek
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
http://localhost/t...ganizacniJednotka	14740

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