About: Random mutagenesis of lectins and high-throughput screening of mutant libraries

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. Random mutagenesis includes a lot of methods used to create diverse mutant libraries and to screen these libraries. In our work two methodical directions are being applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA. Mutational reaction conditions can support even higher number of random substitutions. Targeted-random mutagenesis is being performed by mixture of special oligonucleotide primers carried random base substitutions on selected site(s). An effective high-throughput screening must be employed while large mutant libraries are produced by random mutagenesis techniques. Currently, methods of high-throughput screening have been optimized to maximize of automation of this process. Several approaches simplifying the protein mixture for binding studies were applied. Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. Random mutagenesis includes a lot of methods used to create diverse mutant libraries and to screen these libraries. In our work two methodical directions are being applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA. Mutational reaction conditions can support even higher number of random substitutions. Targeted-random mutagenesis is being performed by mixture of special oligonucleotide primers carried random base substitutions on selected site(s). An effective high-throughput screening must be employed while large mutant libraries are produced by random mutagenesis techniques. Currently, methods of high-throughput screening have been optimized to maximize of automation of this process. Several approaches simplifying the protein mixture for binding studies were applied. (en)
Title	Random mutagenesis of lectins and high-throughput screening of mutant libraries Random mutagenesis of lectins and high-throughput screening of mutant libraries (en)
skos:prefLabel	Random mutagenesis of lectins and high-throughput screening of mutant libraries Random mutagenesis of lectins and high-throughput screening of mutant libraries (en)
skos:notation	RIV/00216224:14740/11:00049899!RIV12-GA0-14740___
http://linked.open...avai/predkladatel	Středoevropský technologický institut
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA303/09/1168), P(GD301/09/H004), P(LC06030), S, Z(MSM0021622413)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Pokorná, Martina Wimmerová, Michaela Mrázková, Jana
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	225679
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/11:00049899
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	lectin; PA-IIL; random mutagenesis; high-throughput screening; targeted-random mutagenesis (en)
http://linked.open.../riv/klicoveSlovo	lectin PA-IIL targeted-random mutagenesis high-throughput screening random mutagenesis
http://linked.open...ontrolniKodProRIV	[B96A802CC673]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Lectins from human pathogens - structure, function, engineering Molecular and structural biology of selected antitumor drugs. From mechanistic studies to chemotherapy of tumors Biomolecular centre
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Mrázková, Jana Pokorná, Martina Wimmerová, Michaela
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
http://localhost/t...ganizacniJednotka	14740

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