About: Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils?

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Isolation of granulocyte cells from blood is necessary for accurate studying of changes in their expression. After gradient centrifugation, we obtain relatively pure granulocyte populations with different ratios of neutrophils and eosinophils. Unfortunately, in many studies in this field the expression results are not set according to the real variability of the granulocyte population. In many cases, the granulocyte population is marked simply as neutrophils and the residual population of eosinophils is not considered. Based on our recent study where we tracked the general transcription factor RNA polymerase II (RNAP II), we hypothesized that eosinophils are more transcriptionally active cells than neutrophils. We decided to test our hypothesis on isolated cells because its implications could change our view on many past expression analyses performed on granulocytes. In our experiments, we isolated neutrophils and eosinophils and measured their total RNA production. Isolation of granulocyte cells from blood is necessary for accurate studying of changes in their expression. After gradient centrifugation, we obtain relatively pure granulocyte populations with different ratios of neutrophils and eosinophils. Unfortunately, in many studies in this field the expression results are not set according to the real variability of the granulocyte population. In many cases, the granulocyte population is marked simply as neutrophils and the residual population of eosinophils is not considered. Based on our recent study where we tracked the general transcription factor RNA polymerase II (RNAP II), we hypothesized that eosinophils are more transcriptionally active cells than neutrophils. We decided to test our hypothesis on isolated cells because its implications could change our view on many past expression analyses performed on granulocytes. In our experiments, we isolated neutrophils and eosinophils and measured their total RNA production. (en)
Title	Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils? Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils? (en)
skos:prefLabel	Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils? Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils? (en)
skos:notation	RIV/00216224:14330/10:00067250!RIV14-MSM-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06052), Z(MSM0021622419), Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Ručka, Zdeněk Stejskal, Stanislav Krontorád Koutná, Irena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	265016
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/10:00067250
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	total RNA; gradient centrifugation; cell isolation; magnetic separation; gene expression; expression activity; neutrophils; eosinophils; granulocytes (en)
http://linked.open.../riv/klicoveSlovo	gene expression neutrophils cell isolation expression activity total RNA gradient centrifugation magnetic separation granulocytes eosinophils
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[5234044AFEC6]
http://linked.open...i/riv/nazevZdroje	FOLIA BIOLOGICA
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Stejskal, Stanislav Krontorád Koutná, Irena Ručka, Zdeněk
http://linked.open...ain/vavai/riv/wos	000286039500003
http://linked.open...n/vavai/riv/zamer	Highly Parallel and Distributed Computing Systems Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	0015-5500
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	14330

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