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  • In our analyses we combined molecular and computational approaches to understand the mutant p53 function in specific gene regulation via binding to non-canonical DNA structures in chromatin DNA. We used two glioblastoma cell lines U251 (R273H) and Onda11 (R273C) expressing endogenous mutp53 proteins to isolate natural mutant p53 binding sites (mutp53BS) by genome- wide ChIP-cloning. In our computational work, we developed tools for rapid identification of DNA sequences (among the isolated mutp53BS) tending to form non-B structures (hairpins and triplex DNA) as well as for mapping their genomic locations. These sites are frequently localized in the regulatory first introns of genes and are enriched in repetitive elements. Potential to form triplex and cruciform structures was predicted by developed computational tools and detected by enzymatic and chemical probing.
  • In our analyses we combined molecular and computational approaches to understand the mutant p53 function in specific gene regulation via binding to non-canonical DNA structures in chromatin DNA. We used two glioblastoma cell lines U251 (R273H) and Onda11 (R273C) expressing endogenous mutp53 proteins to isolate natural mutant p53 binding sites (mutp53BS) by genome- wide ChIP-cloning. In our computational work, we developed tools for rapid identification of DNA sequences (among the isolated mutp53BS) tending to form non-B structures (hairpins and triplex DNA) as well as for mapping their genomic locations. These sites are frequently localized in the regulatory first introns of genes and are enriched in repetitive elements. Potential to form triplex and cruciform structures was predicted by developed computational tools and detected by enzymatic and chemical probing. (en)
Title
  • Recognition of non-canonical DNA structures in genomic DNA sequences by p53 proteins
  • Recognition of non-canonical DNA structures in genomic DNA sequences by p53 proteins (en)
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  • Recognition of non-canonical DNA structures in genomic DNA sequences by p53 proteins
  • Recognition of non-canonical DNA structures in genomic DNA sequences by p53 proteins (en)
skos:notation
  • RIV/00216224:14330/09:00048866!RIV11-GA0-14330___
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  • P(GA204/08/1560)
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  • 338373
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  • RIV/00216224:14330/09:00048866
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  • p53; triplex DNA; DNA-binding; gene regulation (en)
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  • [B420561C6C19]
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  • Fojta, Miroslav
  • Lexa, Matej
  • Navrátilová, L.
  • Deppert, W.
  • Brazdova, Marie
  • Kyjovsky, I.
  • Paleček, E.
  • Tichy, V.
http://localhost/t...ganizacniJednotka
  • 14330
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