Attributes | Values |
---|
rdf:type
| |
Description
| - The nuclear architecture of selected chromosomes in apoptotic nuclei of human leukemic cells K-562 and HL-60 was investigated in the presented paper. Etoposide and prolonged confluence were used for the induction of apoptosis. DAPI- as well as TUNEL- labelling of apoptotic nuclear bodies was combined with visualisation of chromosomal territories by the FISH technique. Simultaneous vital staining by Annexin V, PI and Hoechst 33342 was applied in order to distinguish apoptotic, necrotic and intact cell fraction of tested population. Our FISH analyses revealed that the 3D- structure of apoptotic nuclei as well as the 3D- structure of apoptotic bodies is preserved in formaldehyde fixed cells. High molecular weight DNA fragmentation was determined in apoptotic K-562 cells in contrast to oligonucleosomal cleavage observed in apoptotic HL In comparison with the control, an increased number of centromeric FISH signals were observed in prolonged confluence treated K-562 cells induced to apoptosis.
- The nuclear architecture of selected chromosomes in apoptotic nuclei of human leukemic cells K-562 and HL-60 was investigated in the presented paper. Etoposide and prolonged confluence were used for the induction of apoptosis. DAPI- as well as TUNEL- labelling of apoptotic nuclear bodies was combined with visualisation of chromosomal territories by the FISH technique. Simultaneous vital staining by Annexin V, PI and Hoechst 33342 was applied in order to distinguish apoptotic, necrotic and intact cell fraction of tested population. Our FISH analyses revealed that the 3D- structure of apoptotic nuclei as well as the 3D- structure of apoptotic bodies is preserved in formaldehyde fixed cells. High molecular weight DNA fragmentation was determined in apoptotic K-562 cells in contrast to oligonucleosomal cleavage observed in apoptotic HL In comparison with the control, an increased number of centromeric FISH signals were observed in prolonged confluence treated K-562 cells induced to apoptosis. (en)
|
Title
| - Architecture of chromosomal territories in apoptotic cells
- Architecture of chromosomal territories in apoptotic cells (en)
|
skos:prefLabel
| - Architecture of chromosomal territories in apoptotic cells
- Architecture of chromosomal territories in apoptotic cells (en)
|
skos:notation
| - RIV/00216224:14330/03:00008243!RIV11-AV0-14330___
|
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| - P(GA301/01/0186), P(IAB5004102), P(IBS5004010), Z(MSM 143300002)
|
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14330/03:00008243
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - human genome structure (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...v/mistoKonaniAkce
| |
http://linked.open...i/riv/mistoVydani
| |
http://linked.open...i/riv/nazevZdroje
| |
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...iv/tvurceVysledku
| - Bártová, Eva
- Kozubek, Stanislav
- Matula, Pavel
- Souček, Karel
- Fojtová, Miloslava
- Palša, Stanislav
|
http://linked.open...vavai/riv/typAkce
| |
http://linked.open.../riv/zahajeniAkce
| |
http://linked.open...n/vavai/riv/zamer
| |
number of pages
| |
http://purl.org/ne...btex#hasPublisher
| |
https://schema.org/isbn
| |
http://localhost/t...ganizacniJednotka
| |