About: Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles

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About: Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The paper presents the development of an advanced extraction and fast analytical LC MS/MS method for simultaneous analyses of reduced and oxidized glutathione (GSH and GSSG, respectively) in different animal tissues. The simultaneous determination of GSH and GSSG is crucial because the amount and ratio of both GSH and GSSG may be altered in response to oxidative stress, an important mechanism of toxicity. The method uses the derivatization of free thiol groups in GSH. Its performance was demonstrated for less explored tissues (lung, brain, and liver) in mouse. The combined extraction and analytical method has very low variability and good reproducibility, maximum coefficients of variance for within-run and between-run analyses under 8 %, and low limits of quantification; for GSH and GSSG, these were 0.2 nM (0.06 ng/mL) and 10 nM (6 ng/mL), respectively. The paper presents the development of an advanced extraction and fast analytical LC MS/MS method for simultaneous analyses of reduced and oxidized glutathione (GSH and GSSG, respectively) in different animal tissues. The simultaneous determination of GSH and GSSG is crucial because the amount and ratio of both GSH and GSSG may be altered in response to oxidative stress, an important mechanism of toxicity. The method uses the derivatization of free thiol groups in GSH. Its performance was demonstrated for less explored tissues (lung, brain, and liver) in mouse. The combined extraction and analytical method has very low variability and good reproducibility, maximum coefficients of variance for within-run and between-run analyses under 8 %, and low limits of quantification; for GSH and GSSG, these were 0.2 nM (0.06 ng/mL) and 10 nM (6 ng/mL), respectively. (en)
Title	Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles (en)
skos:prefLabel	Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles (en)
skos:notation	RIV/00216224:14310/14:00079120!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(GAP503/11/2315), P(LO1214), S
http://linked.open...iv/cisloPeriodika	24
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Lebedová, Jana Bláha, Luděk Hilscherová, Klára Bláhová, Lucie Kohoutek, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	45046
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00079120
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Glutathione; LC MS/MS; Tissues; Oxidative stress; Nanoparticle; Cadmium oxide (en)
http://linked.open.../riv/klicoveSlovo	Tissues Cadmium oxide LC MS/MS Oxidative stress Nanoparticle Glutathione
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[A7B86BF8E594]
http://linked.open...i/riv/nazevZdroje	Analytical and Bioanalytical chemistry
http://linked.open...in/vavai/riv/obor	DN
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs Centrum pro výzkum toxických látek v prostředí
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	406
http://linked.open...iv/tvurceVysledku	Bláha, Luděk Hilscherová, Klára Míšek, Ivan Večeřa, Z. Kohoutek, Jiří Bláhová, Lucie Buchtova, M. Lebedová, Jana
http://linked.open...ain/vavai/riv/wos	000341834500013
issn	1618-2642
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00216-014-8033-z
http://localhost/t...ganizacniJednotka	14310

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