About: Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria, Photorhabdus luminescens and transformed Escherichia coli, which can be used as a real-time measurement of antibacterial activity in insect haemolymph. This method is based on the production of the bioluminescence signal depending on the viability of bacterial cells. We observed a significant rapid dose-dependent decrease in bioluminescence using both bacterial species, and Bombyx mori or Galleria mellonella haemolymph, which was confirmed by the decrease in bacterial viability determined by plating. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed for haemolymph plasma without haemocytes. Antibacterial activity directed against Gram negative bacteria was recorded in unaffected insect larvae as well as after septic injury; increased antibacterial activity of haemolymph was detected in the latter case confirming the inducibility of antimicrobial agents. We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria, Photorhabdus luminescens and transformed Escherichia coli, which can be used as a real-time measurement of antibacterial activity in insect haemolymph. This method is based on the production of the bioluminescence signal depending on the viability of bacterial cells. We observed a significant rapid dose-dependent decrease in bioluminescence using both bacterial species, and Bombyx mori or Galleria mellonella haemolymph, which was confirmed by the decrease in bacterial viability determined by plating. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed for haemolymph plasma without haemocytes. Antibacterial activity directed against Gram negative bacteria was recorded in unaffected insect larvae as well as after septic injury; increased antibacterial activity of haemolymph was detected in the latter case confirming the inducibility of antimicrobial agents. (en)
Title	Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph (en)
skos:prefLabel	Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph (en)
skos:notation	RIV/00216224:14310/14:00073253!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(EE2.3.30.0009), P(GP206/09/P470), P(QJ1210047)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Vojtek, Libor Hyršl, Pavel Dobeš, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	5427
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00073253
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Antibacterial activity; antimicrobial peptides; Lepidoptera; bioluminescent bacteria; Bombyx mori; Escherichia coli; Galleria mellonella; Photorhabdus luminescens (en)
http://linked.open.../riv/klicoveSlovo	antimicrobial peptides Galleria mellonella bioluminescent bacteria Antibacterial activity Escherichia coli Lepidoptera Photorhabdus luminescens Bombyx mori
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[51ACC2ABEAA2]
http://linked.open...i/riv/nazevZdroje	European Journal of Entomology
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	The influence of the eicosanoid biosynthesis inhibitors to immunity of the wax moth Galleria mellonella Employment of Newly Graduated Doctors of Science for Scientific Excellence Research and development of novel agents for honey bee health improvement
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	111
http://linked.open...iv/tvurceVysledku	Dobeš, Pavel Hyršl, Pavel Vojtek, Libor Atosuo, Janne Büyükgüzel, Ender
issn	1210-5759
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.14411/eje.2014.045
http://localhost/t...ganizacniJednotka	14310

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