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  • Innate immunity of both vertebrates and invertebrates contents number of antibacterial mechanisms. In invertebrates there is lysozyme and several different antibacterial peptides which are divided according to their stucture to four main groups called linear a-helical, cysteine-stabilized, proline-rich and glycine-rich inducible antimicrobial peptides. In vertebrates antibacterial components cooperate with antibodies, complement cascade and myeloperoxidase activity. Nowadays, many common methods for determination of non-specific innate immune parameters are known in vertebrates, nevertheless there is not so large variety of them in invertebrates. Therefore we tried to apply to invertebrates the bioluminescent bacteria-based assay which is commonly used for complement determination in vertebrates. Bioluminescent bacteria are frequent in salt water environment, but there is the only one genus of soil bacteria with natural bioluminescence – Photorhabdus sp.
  • Innate immunity of both vertebrates and invertebrates contents number of antibacterial mechanisms. In invertebrates there is lysozyme and several different antibacterial peptides which are divided according to their stucture to four main groups called linear a-helical, cysteine-stabilized, proline-rich and glycine-rich inducible antimicrobial peptides. In vertebrates antibacterial components cooperate with antibodies, complement cascade and myeloperoxidase activity. Nowadays, many common methods for determination of non-specific innate immune parameters are known in vertebrates, nevertheless there is not so large variety of them in invertebrates. Therefore we tried to apply to invertebrates the bioluminescent bacteria-based assay which is commonly used for complement determination in vertebrates. Bioluminescent bacteria are frequent in salt water environment, but there is the only one genus of soil bacteria with natural bioluminescence – Photorhabdus sp. (en)
Title
  • Antibacterial agents in innate immunity: comparison of vertebrates and invertebrates
  • Antibacterial agents in innate immunity: comparison of vertebrates and invertebrates (en)
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  • Antibacterial agents in innate immunity: comparison of vertebrates and invertebrates
  • Antibacterial agents in innate immunity: comparison of vertebrates and invertebrates (en)
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  • RIV/00216224:14310/11:00049851!RIV12-GA0-14310___
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  • P(GP206/09/P470)
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  • 186400
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  • RIV/00216224:14310/11:00049851
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  • vertebrates; invertebrates; Escherichia coli K12; Photorhabdus luminescens; bioluminescence; antibacterial peptides; antibacterial assay (en)
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  • [39094C7D1349]
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  • Dobeš, Pavel
  • Hyršl, Pavel
  • Vojtek, Libor
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  • 14310
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