About: Antibacterial methods based on bioluminescent bacteria

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Bioluminescent bacteria are common in salt water environment, but there is the only one genus of soil bacteria with natural bioluminescence – Photorhabdus sp. These symbiotic bacteria reside the gut of entomopathogenic nematodes which are obligate insect parasites with the increasing importance as biological control agent. Isolated Photorhabdus luminescens was used separately for determination of pathogenity to insects. Except P. luminescens the artificial bioluminescent bacterium was used - genetically modified Escherichia coli K12 that carries Photorhabdus genes for bioluminescence. Both of these G- bacteria can be used for antibacterial assays based on their bioluminescence ability. Bioluminescence reaction is mediated by bacterial enzyme luciferase which catalyses the oxidation of long-chain aldehyde (substrate) and reduces flavin mononucleotide with emission of light. This emission can be immediately measured by the luminometer. Bioluminescent bacteria are common in salt water environment, but there is the only one genus of soil bacteria with natural bioluminescence – Photorhabdus sp. These symbiotic bacteria reside the gut of entomopathogenic nematodes which are obligate insect parasites with the increasing importance as biological control agent. Isolated Photorhabdus luminescens was used separately for determination of pathogenity to insects. Except P. luminescens the artificial bioluminescent bacterium was used - genetically modified Escherichia coli K12 that carries Photorhabdus genes for bioluminescence. Both of these G- bacteria can be used for antibacterial assays based on their bioluminescence ability. Bioluminescence reaction is mediated by bacterial enzyme luciferase which catalyses the oxidation of long-chain aldehyde (substrate) and reduces flavin mononucleotide with emission of light. This emission can be immediately measured by the luminometer. (en)
Title	Antibacterial methods based on bioluminescent bacteria Antibacterial methods based on bioluminescent bacteria (en)
skos:prefLabel	Antibacterial methods based on bioluminescent bacteria Antibacterial methods based on bioluminescent bacteria (en)
skos:notation	RIV/00216224:14310/11:00049709!RIV12-GA0-14310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GP206/09/P470)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Vojtek, Libor Hyršl, Pavel Dobeš, Pavel
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	186403
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/11:00049709
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Antibacterial assay; Bioluminescence; Escherichia coli; Photorhabdus luminescens (en)
http://linked.open.../riv/klicoveSlovo	Antibacterial assay Bioluminescence Escherichia coli Photorhabdus luminescens
http://linked.open...ontrolniKodProRIV	[EEC8EE136FDC]
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	The influence of the eicosanoid biosynthesis inhibitors to immunity of the wax moth Galleria mellonella
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Dobeš, Pavel Hyršl, Pavel Vojtek, Libor
http://localhost/t...ganizacniJednotka	14310

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